We narrowed to 12,598 results for: 112
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Plasmid#166861PurposeExpression of codon optimized SARS-COV-2 Nsp8 in E. coliDepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only
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ZJOM70
Plasmid#133658PurposeIntegration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inmCherry
Plasmid#60239PurposeHIV-1 transfer vector that encodes mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseLentiviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLP700
Plasmid#203742PurposeC-terminal tagging of target proteins with AID*/3xHA degronDepositorInsertIAA17 auxin binding domain codons 71-114
UsePcr amplification template for gene taggingTags3xHAPromoternoneAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP701
Plasmid#203743PurposeC-terminal tagging of target proteins with 3xV5-IAA17 degronDepositorInsertIAA17 auxin binding domain
UsePcr amplification template for gene taggingTags3xV5PromoternoneAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
TagsTwinStrepExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-mBoNT/F
Plasmid#168015PurposeExpresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cellsDepositorInsertMBP-BoNT/F-LC(1-428)-6xHis
TagsHisx6 and maltose binding proteinExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
ExpressionBacterialAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PssaG-GFPLVA
Plasmid#23343DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA
Plasmid#51473PurposeBicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.DepositorInsertmRuby2-P2A-GCaMP6m
UseAAVPromoterhSyn1Available SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVG1
Plasmid#111444PurposeUnified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempateDepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
ExpressionMammalianPromoterT7 PromoterAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf R401H
Plasmid#118334PurposeExpression of human CrafDepositorAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1184
Plasmid#205965PurposeNZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)DepositorInsertDinG HNH
Tags14xHis-TwinStrep-bdSUMOExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAceR
Plasmid#195521PurposeRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoterDepositorInsertpAceR
UseAAVExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inGFPt
Plasmid#60237PurposeHIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intronDepositorInsertinGFPt
UseLentiviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-WW-CIB1-biLINuS
Plasmid#171757PurposeLentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuSDepositorInsertSynthetic gene containing LexA-WW-CIB1-biLINuS
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-Halo-CAMSAP3
Plasmid#191329PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertHalo-CAMSAP3 (Camsap3 Mouse)
ExpressionMammalianAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf SSDD
Plasmid#118337PurposeExpression of human CrafDepositorAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G2
Plasmid#40178DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ub[9]-mRFP[1]
Plasmid#20249DepositorInsertubiquitin C (UBC Human)
TagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycineAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2
Plasmid#161570PurposeConstitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoterDepositorInsertPC 3xFLAG-FKBP-FGFR4-TEVp
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
UKNP6.1.1
Plasmid#98386PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRU5-inmCherry
Plasmid#60238PurposeHTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intronDepositorInsertinmCherry
UseRetroviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …Available SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG4-CMV-luc
Plasmid#46322DepositorInsert5xGal4
UseLuciferaseTagsCMV promoter and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
TagsmNeptuneExpressionWormPromoterPtwk-40Available SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
ExpressionWormPromotereft-3Available SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA E28A,E29A
Plasmid#145785PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).DepositorInsertCapsid
UseLentiviral and RetroviralExpressionMammalianMutationChanged Capsid residues Glutamic Acid residues 28…Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1182
Plasmid#205964PurposeNZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1DepositorInsertDinG HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
tdTomato/pTREX-b
Plasmid#68709PurposeExpresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.DepositorInserttdTomato
UseExpression of tdtomato red fluorescence protein i…Available SinceOct. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP1 eforRed toehold switch
Plasmid#107353PurposeExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressedDepositorInserttoehold switch 1 + eforRed
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiEKAR2G1
Plasmid#40177DepositorInsertErk activity reporter
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EAAARx8-CaaX
Plasmid#155224PurposeMPAct with increased search radiusDepositorInsertF-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
TagsmCitrineExpressionMammalianAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag Nprl2 pLJM1
Plasmid#46338DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOtop1_pGEMHE
Plasmid#114674PurposeThis construct was used to express mouse Otop1 in Xenopus laevis oocytesDepositorInsertOtopetrin 1 (Otop1 Mouse)
UseFor generating the mrna, which can highly express…MutationSNP (rs49325632): Glycine 396 to AlaninePromoterT7Available SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA Q63A,Q67A
Plasmid#145784PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).DepositorInsertCapsid
UseLentiviral and RetroviralExpressionMammalianMutationChanged Capsid residues Glutamines 63 and 67 to a…Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM160
Plasmid#134169PurposeBacterial expression of RB3 stathmin-like domain (SLD)DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAd-Sox2
Plasmid#19767DepositorAvailable SinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only