We narrowed to 16,889 results for: Por

1. AID∆C-Cas9n-UGI-NLS

   Plasmid

   #198890

   Purpose

   Express AID(1-188) BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Actication induced deaminase (AICDA Human)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 189-198

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-PH(VPS13C)

   Plasmid

   #232869

   Purpose

   Expresses the PH domain of VPS13C in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   the PH domain of VPS13C (VPS13C Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-OSBP

   Plasmid

   #232871

   Purpose

   Expresses OSBP in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   mCherry-OSBP (OSBP Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuet-v2 RsTAL At4CL

   Plasmid

   #126524

   Purpose

   expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

   Depositor

   Andrew Woolley

   Article

   Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

   Inserts

   tyrosine ammonia lyase
   p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. OMM-Flag-APEX2-SOPP3

   Plasmid

   #229073

   Purpose

   Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVNP3.0-PEmax

   Plasmid

   #206883

   Purpose

   Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   PEmax
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PGK mScarlet-LC3B

   Plasmid

   #200083

   Purpose

   Mammalian expression of fluorescently-tagged marker for autophagic vesicles

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   Map1lc3b (Map1lc3b Rat)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

   Plasmid

   #171150

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRXIP-GFP-CERT(PHD)

   Plasmid

   #221746

   Purpose

   Stable expression of GFP-CERT(PHD)in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–116

   Promoter

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221_SLC2A2

    Plasmid

    #132126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pDONR221_SLC38A9

    Plasmid

    #132070

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A9 (SLC38A9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid

    #164131

    Purpose

    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-PH-TAPP1

    Plasmid

    #161985

    Purpose

    Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

    Depositor

    David Bryant, Dario Alessi

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-HA-Ubiquitin-K11R

    Plasmid

    #121154

    Purpose

    Mammalian expression of HA tagged ubiquitin K11R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K11 mutated to an R

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

    Plasmid

    #184395

    Purpose

    Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-SLC66A2_STOP

    Plasmid

    #221419

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC66A2 (PQLC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD3-2A_pMI-LO

    Plasmid

    #153418

    Purpose

    Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-Ubiquitin-K27R

    Plasmid

    #121155

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 mutated to an R

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDNR-SpCas9-Gem

    Plasmid

    #80424

    Purpose

    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Gem
    

    Use

    CRISPR

    Tags

    Geminin

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221_SLC22A31

    Plasmid

    #132036

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC22A31 (SLC22A31 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

    Plasmid

    #154769

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y125A

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVNP3.0-ABE8e

    Plasmid

    #206882

    Purpose

    Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVNP3.0-PEmaxRH

    Plasmid

    #206884

    Purpose

    Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    PEMax Delta RNAseH
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPN089

    Plasmid

    #91614

    Purpose

    Express sgRNA targeting human FURIN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    FURIN (FURIN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pDONR221_SLC6A14

    Plasmid

    #131865

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A14 (SLC6A14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pDONR221_SLC2A4

    Plasmid

    #132102

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A4 (SLC2A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. Lenti-pNFkB::Cre-mRuby3

    Plasmid

    #164137

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    NFkB promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NFkB synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHis-LMW_FGF2

    Plasmid

    #157657

    Purpose

    Expresses FGF2 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changed Cystein 211,229 to serine, deleted amino …

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TetO-FUW-MEF2CA

    Plasmid

    #61538

    Purpose

    Expresses MEF2CA in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    MEF2CA (MEF2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pDONR221_SLC5A2

    Plasmid

    #132241

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC5A2 (SLC5A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pRK5-HA-Ubiquitin-K6R

    Plasmid

    #121153

    Purpose

    Mammalian expression of HA tagged ubiquitin K6R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K6 mutated to an R

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRXIB-mRuby3-CERT(PHD)

    Plasmid

    #221762

    Purpose

    Stable expression of mRuby3-CERT(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR221_SLC35A3

    Plasmid

    #132135

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35A3 (SLC35A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pBa.GFP-KIF1Bbeta tail 387-1770

    Plasmid

    #134620

    Purpose

    Mammalian expression of mouse KIF1Bbeta 387-1170

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Bbeta (Kif1b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-Tag4A-MMP8 Wt

    Plasmid

    #29545

    Depositor

    Yardena Samuels

    Article

    Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

    Insert

    Metalloproteinase protein 8 (MMP8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pHR EGFR-SLP76-TagBFP

    Plasmid

    #188630

    Purpose

    Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    SLP76-TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. FKBP-beta2(616-951)-GFP

    Plasmid

    #100726

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Expression

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. Lenti-pHSE::Cre-mRuby3

    Plasmid

    #164136

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    HSE promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSE synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLV-mCherry-hCdt1(1-100)ΔCy

    Plasmid

    #193759

    Purpose

    Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLV-CMV-XRCC1-EGFP-Hygro

    Plasmid

    #176062

    Purpose

    EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONR221_SLC35F2

    Plasmid

    #132121

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35F2 (SLC35F2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

    Plasmid

    #188634

    Purpose

    HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    CRISPR

    Tags

    CD3ε-mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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