162,160 results

1. pKJ0463

   Plasmid

   #228715

   Purpose

   Bacterial expression of epT7 RNAP; for protein production and purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Science 0, eadr6006

   Insert

   Sumo_epT7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T3M/G47A/E643G

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_EGFR_p.ELREA_746del

   Plasmid

   #82911

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, David Root, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Deletion of residues 746-750 (ELREA)

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSIE1

   Plasmid

   #136355

   Purpose

   Selection by Inducible Effectors

   Depositor

   Andrew Goodman

   Article

   Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

   Inserts

   Type VI effector bfe1
   Type VI effector bte1
   BT_4676
   tetR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   ATG removed to add signal sequence before gene

   Promoter

   P1TDP-GHO23

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tet-O-FUW-Brn2

   Plasmid

   #27151

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SmoM2 (W535L)-pcw107-V5

   Plasmid

   #64628

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   W535L

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRES2-EGFP-SHP2WT

   Plasmid

   #12283

   Depositor

   Anton Bennett

   Article

   Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

   Insert

   SHP-2WT (PTPN11 Human)
   

   Use

   Tags

   Myc (not canonical: KLISEEDL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMega-MaPylRS

   Plasmid

   #200225

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   proK-lacO and tacI

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE337

   Plasmid

   #153246

   Purpose

   M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HR_G3BP1-V5-APEX2-GFP

    Plasmid

    #105284

    Purpose

    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
    

    Use

    Human homologous recombination donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FU-H2B-GFP-IRES-Puro

    Plasmid

    #69550

    Purpose

    Lentiviral, selectable fluorescent GFP tagging of nuclei.

    Depositor

    Charles Gersbach

    Insert

    Histone 2B - green fluorescent protein fusion
    

    Use

    Lentiviral

    Tags

    GFP fusion

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Mito-CanlonicSF/pCMV-myc-mito

    Plasmid

    #224465

    Purpose

    To explore mitochondrial lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Rauseo et al bioRxiv 2024.07.19.604361

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF1972

    Plasmid

    #143987

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX2 (TBX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KHBD00727

    Plasmid

    #39748

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9932 (CG9932 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tad-CBE(CCC5)

    Plasmid

    #235073

    Purpose

    Expresses Tad-CBE(CCC5) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCC5)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Tad-CBE(TCA3)

    Plasmid

    #235064

    Purpose

    Expresses Tad-CBE(TCA3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCA3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tad-CBE(TCT1)

    Plasmid

    #235065

    Purpose

    Expresses Tad-CBE(TCT1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCT1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Hy_pUbi-p63e mCherry SV40

    Plasmid

    #176300

    Purpose

    Expresses mCherry mRNA from the Drosophila Ubi-p63e promoter

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-Kl-EGFP

    Plasmid

    #45532

    Depositor

    Zipora Yablonka-Reuveni

    Article

    Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

    Insert

    alpha Klotho (Kl Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLQ14107 fwYellow-targeting sgRNA

    Plasmid

    #239456

    Purpose

    guide for fwYellow

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLQ3533 eforRed

    Plasmid

    #239451

    Purpose

    express eforRed chromoprotein

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    eforRed
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ3534 fwYellow

    Plasmid

    #239452

    Purpose

    express fwYellow chromoprotein

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSLQ14112 tyrosinase-targeting sgRNA

    Plasmid

    #239751

    Purpose

    guide for tyrosinase

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSLQ13960 tyrosinase(Priestia megaterium)

    Plasmid

    #239454

    Purpose

    express tyrosinase protein and melanin

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSLQ14103 aeBlue-targeting_sgRNA

    Plasmid

    #239750

    Purpose

    guide for aeBlue

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting aeBlue
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-MS2-Tet1-CD

    Plasmid

    #83341

    Purpose

    To introduce MS2 coat protein fused Tet1-CD

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    MS2-Tet1-CD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

    Plasmid

    #169186

    Purpose

    To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh and U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SP6-VEE-GFP

    Plasmid

    #58976

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge