We narrowed to 9,353 results for: UTY
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Plasmid#213280PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR32 (GPR32 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR4-DuET
Plasmid#213283PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR4 (GPR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR31-DuET
Plasmid#213279PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR31 (GPR31 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR148-DuET
Plasmid#213266PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR148 (GPR148 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FPR1-DuET
Plasmid#213239PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertFPR1 (FPR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD4-DuET
Plasmid#213230PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD4 (DRD4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR8-DuET
Plasmid#213383PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertTAAR8 (TAAR8 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TAAR9-DuET
Plasmid#213384PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertTAAR9 (TAAR9 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
VIPR2-DuET
Plasmid#213387PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertVIPR2 (VIPR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGDR-DuET
Plasmid#213369PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPTGDR (PTGDR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-noTag (in pLEX_307)
Plasmid#214919Purposeconstitutive expression of SRRD with no tag - used as control for SRRD-HA expressionDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-HA (in pLEX_307)
Plasmid#214918Purposeconstitutive expression of SRRD tagged with HADepositorInsertSRRD (SRRD Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1
Plasmid#208110PurposeExpresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoterDepositorInsertSpG C, U6, Lmna sgRNA1, scaffold
UseAAVTagsExpressionMutationPromoterU6Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1
Plasmid#206974PurposeExpresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoterDepositorInsertNG aa714-1368, U6, Lmna sgRNA1
UseAAVTagsExpressionMutationPromoterU6Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
Plasmid#212805PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-MDmut-Gata2
Plasmid#193091Purposeconstitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cellsDepositorInsertMDmut-Gata2
UseLentiviralTagsExpressionMammalianMutationPromoterUbcAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT021-LAT_HASA-HaloTag-3x FLAG
Plasmid#202505PurposeExpress high ASA Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationSeveral leucine subsitutions in transmembrane dom…PromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔSET+ΔZinc finger domains
Plasmid#193678PurposeConstitutive retroviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseRetroviralTagsExpressionMutationDeletion of SET and Zinc finger domainsPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CPL
Plasmid#131458PurposeEncodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorInsertDYSF (DYSF Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS47A, S65A, T75APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-dVHH[GFP] in pCSDEST
Plasmid#162870PurposeSuper-resolution of GFP in cultureDepositorInsertmEos2-dVHH[EGFP]
UseTagsmEos2ExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX
Plasmid#176121PurposeConstitutivley active, mitochondrial localized Cdc42DepositorInsertCDC42 (CDC42 Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-mTurquoise-RhoA-G14V-deltaCaaX
Plasmid#176123PurposeConstitutively active, mitochondrial localized RhoADepositorInsertRHOA (RHOA Human)
UseTagsExpressionMammalianMutationG14VPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
S113
Plasmid#174551PurposeConstitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)DepositorInsertmdm2 and designed protein LD6 (MDM2 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.mTagBFP2.miR-125b-2
Plasmid#169311PurposeConstitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent proteinDepositorInsertmiR-125b-2 (MIR125B2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3
Plasmid#161572PurposeConstitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoterDepositorInsertTC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM588 EF1α 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA in TUPV2
Plasmid#161564PurposeConstitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM590 EF1α 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA in TUPV2
Plasmid#161566PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B15-VN
Plasmid#135514PurposeMammalian expression of VN-fused and myc-tagged HLA-B*15:01DepositorInsertHLA-B*15:01 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57-VN
Plasmid#135515PurposeMammalian expression of VN-fused and myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd (Y84C/C121S/A139C)
Plasmid#136063PurposeMammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)DepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY84C/C121S/A139CPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8-VN
Plasmid#135511PurposeMammalian expression of VN-fused and myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-CTNNB1_S33Y
Plasmid#133376PurposePiggybac vector for constitutive expressionDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationS33YPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PV259
Plasmid#132340PurposeCasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV503
Plasmid#132342PurposeCasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PV504
Plasmid#132343PurposeCasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertCasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790M_28bp
Plasmid#114721Purpose28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …PromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790M_28bp
Plasmid#114719Purpose4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT2
Plasmid#113093PurposeLuciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationSecond predicted miR-199a-3p binding site mutated…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT-both
Plasmid#113094PurposeLuciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationBoth the predicted miR-199a-3p binding site muta…PromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2741 HC_Kan_EF1ap_p18
Plasmid#100698Purposepart designed to occupy position 18 of EMMA. Functional category: Constitutional promoterDepositorInsertEF1a promoter (EEF1A1 Human)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSM4
Plasmid#101642PurposeEncodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.DepositorInsertKCyG4219
UseTags6XHisExpressionBacterialMutationC-terminal residues (221-223) deletion. Ser220Val…PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_EB
Plasmid#66013PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_FB
Plasmid#66014PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_GB
Plasmid#66015PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only