8,969 results

Showing: 5131 - 5160 of 8969 results

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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Article

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(T17N)

Plasmid

#12601

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (T17N) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLifeAct-mTurquoise2

Plasmid

#36201

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mKO2-SLBP(18-126)

Plasmid

#83914

Purpose

Fluorescent probe for S/G2 cell cycle transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mKO2-SLBP(18-126) (SLBP Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-hUBE1L

Plasmid

#12438

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UBE1L (UBA7 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Article

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Insert

p16 INK4 (CDKN2A Human)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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mCherry-Calreticulin-N-16

Plasmid

#55006

Purpose

Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Calreticulin (CALR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

Plasmid

#100146

Purpose

Expresses CPT1A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

CPT1A (CPT1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-VPS4A

Plasmid

#116924

Purpose

expresses mEGFP-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

silent mutation in position 4059 C to T

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c dom neg

Plasmid

#8885

Depositor

Bruce Spiegelman

Article

Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

Insert

SREBP-1c (Srebf1 Rat)

Expression

Mammalian

Mutation

Y305A amino acid substitution in the active versi…

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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AICSDP-36: AAVS1-mEGFP (PGK)

Plasmid

#114404

Purpose

Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

Depositor

Allen Institute for Cell Science

Insert

AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-BP1-2 pLPC-Puro

Plasmid

#19835

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

Fragment of p53-Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

mCherry

Mutation

Fragment of human 53BP1 aa 1220 - 1711

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pL-CRISPR.EFS.tRFP

Plasmid

#57819

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMT3 p38

Plasmid

#12658

Depositor

John Kyriakis

Insert

p38alpha (MAPK14 Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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HA-TAZ-S89A

Plasmid

#32840

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Tags

HA

Expression

Mammalian

Mutation

S89A

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-DDX4N-mCh-Cry2WT

Plasmid

#101225

Purpose

DDX4(1-236) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

DDX4(1-236)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 synthetic, Human)

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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VHL-pGex2TK

Plasmid

#20790

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

VHL (VHL Human)

Tags

GST

Expression

Bacterial

Mutation

VHL wt cDNA, no mutations

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pET302-6His-dCas9-Halo

Plasmid

#72269

Purpose

Encodes a Halo-labeled fusion of nuclease deficient Cas9

Depositor

Timothée Lionnet

Article

Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

Insert

His6-dCas9-Halo

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-TGFb1

Plasmid

#101762

Purpose

TGFb1 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

transforming growth factor beta 1 promoter (TGFB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT human MyoD-T2A-dsRedExpress2

Plasmid

#60628

Purpose

Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD (MYOD1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector (4A)

Plasmid

#30111

Depositor

Scott Gradia

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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8,969 results