Addgene

We narrowed to 11,177 results for: SHA

Showing: 5191 - 5220 of 11177 results

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pHiUGE V5-epitope donor ORF0

Plasmid

#200377

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc_SV40:PuroR

Plasmid

#183038

Purpose

Expresses the FLAG-tagged OgNLuc (Orange LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACS130

Plasmid

#204595

Purpose

Histone H3.1K9M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 (H3C3 Human)

Use

Lentiviral

Tags

human histone H3.1K9M-V5

Expression

Mammalian

Mutation

H3.1K9M

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pETa(+) HpCsd3 (42-403)

Plasmid

#71285

Purpose

Expresses Csd3 from Helicobacter pylori

Depositor

SeWon Suh

Article

An et al Acta Crystallogr D Biol Crystallogr. 201

Insert

HP0506 (HP0506 )

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
p4456 FLAG-hPLIC-2 NTF

Plasmid

#8662

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal fragment: amino acids 1-107 of hPLIC-2

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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tdKatushka2-VSVG-16

Plasmid

#56052

Purpose

Localization: Golgi/ER Transport, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VSVG

Use

Tags

tdKatushka2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pENTR-PKCG

Plasmid

#16180

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

PRKC gamma (PRKCG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pMZ1001

Plasmid

#204398

Purpose

A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

quorum sensing regulator RpaR (rpaR )
GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PftsH and Synthetic promoter PRpa0002

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 HL1gR4

Plasmid

#124450

Purpose

Expresses gRNA HL1 gR4 in the pSB700 backbone

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

HL1 gR4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-YL1

Plasmid

#15333

Depositor

Ronald Conaway, Joan Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pHiUGE V5-epitope donor ORF2

Plasmid

#200379

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-MannII-N-10

Plasmid

#55903

Purpose

Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Use

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLenti_CMV:Flag-CeNLuc_SV40:PuroR

Plasmid

#183036

Purpose

Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFA-LEU2-I-SceI

Plasmid

#67595

Purpose

I-SceI expression regulated by tetracycline inducible system in S. pombe

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

nmt terminator
I-SceI
TetR

Use

Gene targeting in yeast

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9B

Plasmid

#35919

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Use

Tags

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGL1.7XX

Plasmid

#49344

Purpose

luciferase reporter with mouse Bmp2 promoter

Depositor

Melissa Rogers

Article

Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

Insert

Bmp2 distal promoter (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

nt –1237 to +471

Promoter

Bmp2 distal promoter

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11-V5

Plasmid

#35941

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSper4 cyto roGFP2-Orp1

Plasmid

#64998

Purpose

Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Use

Tags

roGFP2

Expression

Insect

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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U6b-T

Plasmid

#117210

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6b promoter-gRNA

Use

Synthetic Biology

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE flag M33

Plasmid

#1951

Depositor

Robert Kingston

Article

Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

Insert

M33 (Cbx2 Mouse)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-EGFP-WPRE

Plasmid

#187103

Purpose

Double floxed EGFP under the control of EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4-V5

Plasmid

#35929

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSLC-239

Plasmid

#73193

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-yhaV (yhaV )

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Clover-Lysosomes-20

Plasmid

#56528

Purpose

Localization: Lysosome Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHiUGE Myc-epitope donor ORF0

Plasmid

#200374

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE Myc-epitope donor ORF1

Plasmid

#200375

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge