159,557 results

1. pcDNA4/TO-cyclin E

   Plasmid

   #216508

   Purpose

   Expresses cyclin E1 in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CCNE1 (CCNE1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRUPATH Triple Gai2

   Plasmid

   #196049

   Purpose

   Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai2-RLuc8 (GNAI2 Human)
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. acGFP WC visual barcode

   Plasmid

   #158672

   Purpose

   tagging clonal populations with whole cell acGFP fluorescent protein

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   acGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b-csgA

   Plasmid

   #89581

   Purpose

   Express csgA curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgA (csgA )
   

   Use

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-his-LbCpf1-EC

   Plasmid

   #79008

   Purpose

   Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   LbCpf1
   

   Use

   Tags

   MBP-6xHis-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2B-56

   Plasmid

   #177925

   Purpose

   Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   V9I; F67S; G72P, F86Y; R171K

   Promoter

   MYO2

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ac5-STABLE2-puro

   Plasmid

   #208032

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMVTO-HA-GFP

   Plasmid

   #179912

   Purpose

   Constitutively secreted GFP

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. moxGFP

    Plasmid

    #68070

    Purpose

    C-terminal fusion to moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ITSN-mcherry-sspB

    Plasmid

    #85220

    Purpose

    Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    ITSN1(1225-1575) (ITSN1 Human)
    mcherry
    sspB
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    R73Q

    Promoter

    CMV

    Available since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. scFLARE1

    Plasmid

    #158738

    Purpose

    Mammalian expression of scFLARE1 component

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE1
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SEP-GluA1 TKIT

    Plasmid

    #169441

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPC-JAG1-mVenus

    Plasmid

    #171170

    Purpose

    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    JAG1 (JAG1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0424

    Plasmid

    #142262

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PBX3 (PBX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIV-dTomato

    Plasmid

    #21374

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Ub

    Plasmid

    #11928

    Purpose

    Used for in vivo monitoring of ubiquitin localization.

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLostfoX-Kan

    Plasmid

    #99865

    Purpose

    TfoX induction vector for chitin-pathway transformation (KanR)

    Depositor

    Mark Mandel

    Article

    Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

    Insert

    tfoXVF
    

    Use

    V. fischeri suicide cloning vector, cmr, tcr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKEN GFP mut2

    Plasmid

    #20409

    Depositor

    Stanley Falkow, Raphael Valdivia

    Article

    Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S65A V68L S72A

    Promoter

    Available since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eGFPC1 FKBP

    Plasmid

    #67529

    Purpose

    Expresses GFP fused FKBP

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Insert

    FKBP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F36V

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-BAZ1B

    Plasmid

    #65372

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1B (BAZ1B Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VAMP2-pHmScarlet

    Plasmid

    #166890

    Purpose

    For vesicle exocytosis visualization using pHmScarlet

    Depositor

    Pingyong Xu

    Article

    Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

    Insert

    VAMP2 (Vamp2 Mouse)
    

    Use

    Tags

    pHmScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hCEP120-EGFP

    Plasmid

    #50382

    Purpose

    Over-expression of EGFP-tagged hCEP120

    Depositor

    Tang Tang

    Article

    Lin et al J Cell Biol. 2013 Jul 22;202(2):211-9. d

    Insert

    Human CEP120 (CEP120 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Use

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAK_DR30_CasRx_GFP

    Plasmid

    #134843

    Purpose

    All in one overexpression of CasRx and guideRNA

    Depositor

    Michael Yaffe

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    T2A EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-FLEX-GFP

    Plasmid

    #28304

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-CMV-puro_FlnaABD-TS-16flag

    Plasmid

    #234868

    Purpose

    Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AiP14573: pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA (Alias: CN4573)

    Plasmid

    #230871

    Purpose

    AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    FlpO
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)

    Plasmid

    #214855

    Purpose

    Expression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    FlpO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAKlux2

    Plasmid

    #14080

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Bacterial luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac, T3

    Available since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET30(a)-AtNUDX23

    Plasmid

    #175021

    Purpose

    Purification of AtNUDX23 enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    AtNUDX23 (NUDX23 Mustard Weed)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFEB promoter-luciferase reporter

    Plasmid

    #66801

    Purpose

    Reporter for activity of TFEB gene promoter

    Depositor

    Albert La Spada

    Article

    Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

    Insert

    TFEB promoter (Tfeb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PBCAG-eGFP

    Plasmid

    #40973

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Inserts

    PiggyBac transposon 3' terminal repeats
    PiggyBac 5' terminal repeats
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge