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Showing: 6921 - 6960 of 157724 results

  2. AAV-CAG-jGCaMP8f-WPRE

    Plasmid
    #179254
    Purpose
    AAV-mediated expression of GCaMP8f under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179254-plasmid-map-sequence-id-381183

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  3. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    Non-targeting sgRNA human (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71830-plasmid-map-sequence-id-131025

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  8. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid
    #188383
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188383-plasmid-map-sequence-id-371054

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  16. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

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  18. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  20. pOPINK

    Plasmid
    #41143
    Depositor
    Ray Owens
    Insert
    GST
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41143-plasmid-map-sequence-id-54743

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  30. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  31. MC3R-Tango

    Plasmid
    #66429
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC3R (MC3R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. pE5n

    Plasmid
    #17459
    Purpose
    modified pENTR vector for N-terminal ECFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17459-plasmid-map-sequence-id-8627

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  34. pEG302 22aa SunTag NtDRMcd nog

    Plasmid
    #115488
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    115488-plasmid-map-sequence-id-237370

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  37. pRosetta

    Plasmid
    #59700
    Purpose
    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.
    Depositor
    John Doench, David Root
    Inserts
    Puro
    BlastR
    EGFP
    Use
    Lentiviral
    Tags
    2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    59700-plasmid-map-sequence-id-140560

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  38. pGEX4T-1-Optimized UBE1L

    Plasmid
    #165099
    Purpose
    ecoli optimized expression of GST-fusion of human Ube1L
    Depositor
    Arthur Haas
    Insert
    UbeE1L (UBA7 Human)
    Use
    Tags
    GST from plasmid.
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165099-plasmid-map-sequence-id-327741

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Showing: 6921 - 6960 of 157724 results