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pIVT-EGFP-mouseCENP-C

Plasmid

#174444

Purpose

in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, alpha6 [N229A/32R]

Plasmid

#164155

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, alpha6 (Mouse). Derived from hybridoma N229A/32.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, alpha6 (Mus musculus) recombinant mouse monoclonal antibody (Gabra6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR mouse Dicer3

Plasmid

#14781

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Dicer shRNA (Dicer1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pMRX-IP/SECFP-mDFCP1

Plasmid

#58765

Purpose

Expresses SECFP-mDFCP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

SECFP

Expression

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL2B -158/+64

Plasmid

#24946

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT metaxin 2

Plasmid

#12419

Depositor

Paul Bornstein

Insert

metaxin 2 (Mtx2 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only
pRetroX-TRE3G_NT-GSDMD_I105N_

Plasmid

#201174

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Tags

Expression

Mutation

aa1-276 only, I105N

Promoter

TRE3G

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pKS913.CB18.31

Plasmid

#69569

Purpose

TCR beta chain genomic transgenic construct for creating OT-1 mice

Depositor

Francis R. Carbone

Article

Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

Insert

TCR beta (Tcrb Mouse)

Use

Mouse Targeting

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-375

Plasmid

#26336

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-375 (Mir375 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pbs/pU6-HDAC4 RNAi

Plasmid

#21100

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

RNAi against histone deacetylase4 (HDAC4 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGL3Basic-Mdm2-T2

Plasmid

#32366

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) mouse Egr4

Plasmid

#108096

Purpose

Expresses mouse Egr4 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin-H-18

Plasmid

#56448

Purpose

Localization: Talin Head Domain, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

Talin H

Use

Tags

EGFP

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pH1-shOPA1-pSyn-tdtomato

Plasmid

#206316

Purpose

Knocking-down OPA1 in mouse cells

Depositor

Yusuke Hirabayashi

Article

Suga et al PLoS Biol. 2023 Aug 31;21(8):e3002246. d

Insert

shRNA against mouse OPA1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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RRAS1

Plasmid

#55659

Purpose

expression of mouse RRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RRAS1

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

Plasmid

#51255

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Ala

Promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
myc-Rapr-FAK

Plasmid

#25926

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 tr

Plasmid

#14793

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 truncated (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

truncataed HMGA2. As in human tumors, Hmga2-tr la…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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p156 pWZL-EGFP-SLPI

Plasmid

#11069

Depositor

Ramesh Shivdasani

Article

Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

Insert

SLPI (Slpi Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMSCV Cdk2ap1CAN-HA

Plasmid

#178030

Purpose

Retroviral vector for the purpose of overexpression in mammalian cell culture

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

First N-Terminal 27aa are deleted

Promoter

GAG

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_mMSH2

Plasmid

#186156

Purpose

sgRNA targeting murine Msh2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

Msh2_gRNA (Msh2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Synthetic, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

-

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
GST-14-3-3dm (pEBG)

Plasmid

#1943

Depositor

Joseph Avruch

Article

Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Insert

14-3-3 zeta (Ywhaz Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Dimerization-deficient mutant of 14-3-3zeta aa: E…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

Plasmid

#194248

Purpose

Expresses 3 miRNAs targeting mouse alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(mouse) (Snca Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mP58.dJ1-pCDNA3

Plasmid

#21884

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DnaJ domain deleted from C-term

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8a

Plasmid

#42284

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8a (Wnt8a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only