We narrowed to 13,226 results for: 109
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Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFi-P2A-Puro
Plasmid#136902PurposeLentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)DepositorInsertFNLSHiFi
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691APromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
IG301: pMVP (L1-R5) CMV promoter
Plasmid#121692PurposepMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-10
Plasmid#63835PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Tas2r3
Plasmid#48176Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of TAS2R3 (TAS2R3 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH652-RLuc/maxFLuc
Plasmid#29698DepositorInsertFirefly Luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
XE7 Xwnt-8
Plasmid#16869DepositorInsertXwnt-8 RNA (wnt8a.L Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA4CHmneo
Plasmid#69607PurposeExpresses a neomycin tagged codon optimized L1PA4DepositorInsertL1PA4
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MKRN1 WT
Plasmid#78766PurposeTo detect localization of MKRN1 in live mammalian cellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Neo
Plasmid#171684Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)DepositorInsertNeomycin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SMYD2_1
Plasmid#36349DepositorInsertSMYD2 (SMYD2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M2S_AmpR
Plasmid#119154PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts[mCherry]-1A(lacZ)
Plasmid#126980PurposeLevel 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-pheS_NOC
Plasmid#127031PurposeBasic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.DepositorInsertPart PheS_NOC
UseSynthetic BiologyTagsExpressionBacterialMutationpheS A294G promiscuous mutationPromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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IDP424
Plasmid#87018Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsCLIP-Nrx1B-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
minitalin (headR13DD)
Plasmid#182994Purposeexpression of minitalin wt in mammalian cellsDepositorInsertminitalin (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HybridBarcode
Plasmid#174037PurposeFor Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sitesDepositorInsertCRISPR target sites
UseTol2 transgenesisTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/WT SPAST without 5'UTR
Plasmid#87732PurposeExpresses WT spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutation5'UTR deleted (1-190 bp)PromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
IO201: pMVP (R4-R3) mCherry
Plasmid#121732PurposepMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPO640
Plasmid#195296PurposepTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).DepositorInsertminiMcCAST (Tn7575) with KanR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIBR005
Plasmid#177668PurposeConstitutive expression of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIBR001
Plasmid#177628PurposeSIBR Intron 1 (Int1) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
JZ901: pMVP (L5-L4) EF1a promoter
Plasmid#121727PurposepMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NGFP-mAID
Plasmid#140605PurposeExpression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTG01-Amp-FRT
Plasmid#128270PurposePlasmid that produces the following fusion protein: TetR-LOV-TIPDepositorInsertTetR-LOV-TIP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]
Plasmid#71810PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptreCas9-mKate2ps-T1gRNA
Plasmid#62715PurposeInducible; gRNA seq is ATGAGAATCAAGGCGGTCGADepositorInsertCas9–mKate2ps
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
IG001: pMVP (L1-L4) TBG promoter
Plasmid#121689PurposepMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertTBG promoter (Liver specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-sgIGR_dual-MTS-3x-Set I
Plasmid#162764PurposeSeparately expressing 3 unique sgIGR_dual-MTS (SetI)DepositorInsertsgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SEPT11 sh1 shRNA
Plasmid#122020PurposeKnock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.DepositorInsertSEPT11 shRNA (Sept11 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmScarlet-mAID
Plasmid#140618PurposeExpression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
Plasmid#71812PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTH-g
Plasmid#81104PurposeGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_AVR-Pia
Plasmid#123653PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertAVR-Pia
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH16_AVR-Pib_100
Plasmid#121378PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH16_AVR-Pib_100
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH12-rn_00121641
Plasmid#121371PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH12-rn_00121641
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_PH14-rn_00186401
Plasmid#121369PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertPH14-rn_00186401
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquitin K6C
Plasmid#86587PurposeBacterial expression of Ubiquitin K6C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K6CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB38-HF380
Plasmid#37332DepositorInsertCytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
UseDictyostelium expressionTagsHis6 and FLAG tagExpressionMutationdeleted amino acids 1-1387PromoterTRE-PminAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM2_12
Plasmid#183061PurposeControl for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_10
Plasmid#183059PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_9
Plasmid#183058PurposeBiosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locusDepositorInsertsmCherry
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM2_11
Plasmid#183060PurposeControl for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locusDepositorInsertsmCherry
HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterHAA1 and Synthetic promoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only