We narrowed to 21,608 results for: ESC

1. pRU61

   Plasmid

   #167692

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lentiviral-KOR-FRB

   Plasmid

   #191496

   Purpose

   Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

   Depositor

   Wenjing Wang

   Article

   Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

   Insert

   KOR-FRB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFLAG-ING2

   Plasmid

   #13295

   Depositor

   Curtis Harris

   Article

   Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

   Insert

   ING2 (INGX Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pNVLTv2-pvdIJD-Ptrc-base

   Plasmid

   #169243

   Purpose

   Suicide vector for integrating expression cassette in P. putida (IPTG induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJYDN3p

   Plasmid

   #162457

   Purpose

   Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.GFAP.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209662

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZH517

   Plasmid

   #102670

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.hSynap.iGABASnFR.R205A

   Plasmid

   #112162

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.R205A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R205A (no GABA binding)

   Promoter

   hSynapsin

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZR064_CROPseq-sgCD4-CRISPRi

   Plasmid

   #180270

   Purpose

   Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   CD4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. POM42-mCherry

    Plasmid

    #69856

    Purpose

    N-terminal tagging with mCherry using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mCherry minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJT254-litR

    Plasmid

    #202308

    Purpose

    litR repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    litR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAJT249-psrA

    Plasmid

    #202304

    Purpose

    psrA repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    psrA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mIFP24-C1

    Plasmid

    #54820

    Purpose

    Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

    Depositor

    Xiaokun Shu, Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Type

    Empty backbone

    Use

    Tags

    mIFP24

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PEP108E

    Plasmid

    #97394

    Purpose

    binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Use

    Tags

    Plasma membrane target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mTagRFP-T-Vinculin-23

    Plasmid

    #58030

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pET-NtC60αC20NtLC60β

    Plasmid

    #160877

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcL: T7, chaperones: T7

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB2AR-miRFP670nano3

    Plasmid

    #184671

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    B2AR-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    ymVenus

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-GBP1-10gly-lexADBD

    Plasmid

    #50795

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-lexA DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mRuby2-Cx43-7

    Plasmid

    #55894

    Purpose

    Localization: Gap Junctions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Cx43
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-ING2

    Plasmid

    #13294

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pTDpelB-CTwinStrep

    Plasmid

    #45941

    Purpose

    use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    PelB and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFPL-Cg

    Plasmid

    #61652

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCytERM-LSSmGFP

    Plasmid

    #186540

    Purpose

    Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRecLV103-GFP-PGBD5

    Plasmid

    #65409

    Purpose

    Lentiviral expression vector for fusion of GFP with human PGBD5

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.hSynap.iGABASnFR.F102Y.Y137L

    Plasmid

    #112161

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.GFAP.iGABASnFR.F102Y.Y137L

    Plasmid

    #112174

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    GFAP

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGBW-m4046806

    Plasmid

    #145746

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. mEos2-Fibrillarin-7

    Plasmid

    #57377

    Purpose

    Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pSZT025

    Plasmid

    #140033

    Purpose

    KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PEP113E

    Plasmid

    #97399

    Purpose

    binary construct of peroxisome-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Peroxisome target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

    Plasmid

    #162804

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-mCherry2-G

    Plasmid

    #164162

    Purpose

    Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pZH519

    Plasmid

    #102672

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pZH522

    Plasmid

    #102675

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FusionRed-C-Src-7

    Plasmid

    #56107

    Purpose

    Localization: Membrane/Vesicles, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    C-Src
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pMP4516

    Plasmid

    #107775

    Purpose

    Expression of ECFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-ECFP in pBBR1-mcs5
    

    Use

    Tags

    pLacZ-ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. tdKatushka2-VSVG-16

    Plasmid

    #56052

    Purpose

    Localization: Golgi/ER Transport, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    VSVG
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

42. pEB1-mCerulean ME

    Plasmid

    #103971

    Purpose

    Plasmid encoding mCerulean ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean ME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mPapaya1-PDHA1-N-10

    Plasmid

    #56356

    Purpose

    Localization: Mitochondria, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCS4

    Plasmid

    #55751

    Purpose

    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-59 of KanR

    Promoter

    T7-lac

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. p3xFLAG-Tmprss6(Mask)

    Plasmid

    #18790

    Depositor

    Bruce Beutler

    Article

    Du et al Science. 2008 May 23. 320(5879):1088-92.

    Insert

    Type II transmembrane serine protease 6 (Tmprss6 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 567-811 and replaced with 28 …

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

46. pRU52

    Plasmid

    #167678

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSLIK 3xFLAG-MTUS1 zeo

    Plasmid

    #192323

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-MTUS1

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    MTUS1 (MTUS1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. mOrange-H2B-6

    Plasmid

    #57942

    Purpose

    Localization: Nucleus/Histones, Excitation: 548, Emission: 562

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

50. Dendra2-Gamma-Tubulin-17

    Plasmid

    #57721

    Purpose

    Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge