We narrowed to 22,825 results for: Sis

1. APneg-SNAP25d

   Plasmid

   #168014

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   SNAP25-T7PAP
   

   Expression

   Bacterial

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA4854

   Plasmid

   #184849

   Purpose

   Coexpression of PhiC31o recombinase and EGFP

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Inserts

   EGFP
   PhiC31o
   

   Expression

   Mammalian

   Promoter

   CMV and RSV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-FKBP-GFP-HIS3MX6

   Plasmid

   #183023

   Purpose

   PCR-based tagging of yeast

   Depositor

   Santiago Di Pietro

   Article

   Lamb et al STAR Protoc. 2022 Apr 14;3(2):101323. do

   Insert

   FKBP
   

   Use

   Yeast genomic targeting

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PUMA Frag2-Luc

   Plasmid

   #16592

   Depositor

   Bert Vogelstein

   Article

   Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

   Insert

   PUMA promotor fragment (BBC3 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pBABE Hygro ST6GalNaC5

   Plasmid

   #26981

   Depositor

   Joan Massague

   Article

   Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

   Insert

   ST6GalNaC5 (ST6GALNAC5 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. YFP-Rac2(N17)

   Plasmid

   #11398

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Threonine 17 changed to Asparagine.

   Available Since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. UBC13-PGEX4T

   Plasmid

   #25464

   Depositor

   Cynthia Wolberger

   Article

   Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

   Insert

   Ubc13 (UBC13, MMS2 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pAGT5439

   Plasmid

   #192351

   Purpose

   L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-tOCS_CGCT
   

   Expression

   Plant

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ClopHensor-H148G-V224L

   Plasmid

   #46366

   Depositor

   Daniele Arosio

   Article

   Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

   Insert

   ClopHensor-H148G-V224L
   

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCS2+HA-hSeparase

    Plasmid

    #33018

    Depositor

    Marc Kirschner

    Article

    Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

    Insert

    Separase (ESPL1 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. eNme2C-CBE6d

    Plasmid

    #215832

    Purpose

    Express CBE6d (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d-eNme2C-2xUGI
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    hsp-16.48

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti6.2-Renilla-ccdB

    Plasmid

    #87076

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-EGFAB

    Plasmid

    #25770

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

    Insert

    LDL receptor EGFAB (LDLR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pSuperRetropuro-IL-6 shRNA 1

    Plasmid

    #19959

    Depositor

    Christopher Counter

    Article

    Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

    Insert

    pSuperRetropuro-IL-6 shRNA 1 (IL6 Human)
    

    Use

    RNAi and Retroviral

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCBLsgRNA1

    Plasmid

    #199727

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA1 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCBLsgRNA2

    Plasmid

    #199728

    Purpose

    Golden Gate entry vector; 2nd gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA2 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pI6-MTU-DO-URA

    Plasmid

    #160030

    Purpose

    Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyCas9(WT)

    Plasmid

    #101044

    Purpose

    Express Streptococcus pyogenes Cas9

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    SpyCas9
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRetroSuper shSmad4J hygro

    Plasmid

    #19151

    Depositor

    Joan Massague

    Article

    Padua et al Cell. 2008 Apr 4. 133(1):66-77.

    Insert

    small hairpin RNA against SMAD family member 4 (SMAD4 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pAC-EPSILON

    Plasmid

    #53276

    Purpose

    Contains ctrE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and the lcyE gene of Lactuca sativa and thereby produces epsilon-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    lcyE
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2+ Integrin beta 1b-mKate

    Plasmid

    #105975

    Purpose

    in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Tags

    mKate

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET22b-T5-mad32TAG

    Plasmid

    #188997

    Purpose

    Express madanin-1 with TAG at 32 position

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    madanin-1 with TAG at 32 position
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGGE001

    Plasmid

    #48839

    Purpose

    Provides the rbcS terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    rbcS terminator
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBJ1-rat Beta2m

    Plasmid

    #121670

    Purpose

    Expresses rat β-2-microglobulin in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat beta-2- microglobulin (B2m Rat)
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO GFP DNAJB2a

    Plasmid

    #19497

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUb6-Zpo1-V5/His

    Plasmid

    #29323

    Depositor

    Zena Werb

    Article

    Slorach et al Genes Dev. 2011 Mar 1;25(5):471-84. doi:

    Insert

    Zeppo1
    

    Tags

    His and V5

    Expression

    Mammalian

    Available Since

    May 5, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pFA6a-mMaple3-kanMX6

    Plasmid

    #105165

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

    Insert

    mMaple3-kanMX6
    

    Tags

    GlySerGly-mMaple3

    Expression

    Bacterial and Yeast

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPOTv7-blast-tdTomato

    Plasmid

    #179794

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    tdTomato
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSBY2_ligD(FnCpf1)

    Plasmid

    #104623

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    crRNA to ligD
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Pj23119

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-myc3-14-3-3 Beta

    Plasmid

    #19957

    Depositor

    Yue Xiong

    Article

    Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

    Insert

    14-3-3 Protein Beta (YWHAB Human)
    

    Tags

    Myc3

    Expression

    Mammalian

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pPL5628_TEF1*-Cre_MET15

    Plasmid

    #60933

    Purpose

    Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPM2212

    Plasmid

    #167552

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Cosmid

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMSXND1-hPPT1

    Plasmid

    #85817

    Purpose

    Expression of hPPT in mammalian cells

    Depositor

    Sandra Hofmann

    Article

    Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

    Insert

    human PPT1 (PPT1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

37. APneg-SNAP25c

    Plasmid

    #168013

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. APneg-SNAP25b

    Plasmid

    #168012

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBabe-puro-mIR-31

    Plasmid

    #26088

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pLKO.1P shNFS1_1

    Plasmid

    #102963

    Purpose

    Suppression of NFS1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shNFS1_1 (NFS1 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge