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Showing: 851 - 900 of 2919 results

  1. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  2. AAV-U6-gRNA-TnT-Cre

    Plasmid
    #132551
    Purpose
    For loss-of-function experiments in cardiomyocytes
    Depositor
    William Pu
    Inserts
    TnT-Cre
    U6-gRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TnT and U6
    Available since
    Oct. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132551-plasmid-map-sequence-id-258601

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  3. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE and pGK
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  4. pNK-TGCK

    Plasmid
    #45359
    Depositor
    Sean Wu
    Inserts
    tTA
    eGFP-Cre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Nkx2.5 cardiac enhancer and promoter fragme…
    Available since
    Aug. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    45359-plasmid-map-sequence-id-393051

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  6. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  7. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  8. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid
    #59332
    Purpose
    Expresses splitTVA-P2A-EGFP
    Depositor
    Ian Wickersham
    Insert
    splitTVA950-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59332-plasmid-map-sequence-id-92028

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  9. pAAV-synP-FLEX-EGFP-B19G

    Plasmid
    #59333
    Purpose
    Expresses EGFP-P2A-B19G
    Depositor
    Ian Wickersham
    Insert
    EGFP-P2A-B19G
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin-1
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59333-plasmid-map-sequence-id-92029

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  10. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    alpha tubulin T. gondii
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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  11. CAG-FLEX-MCS-WPRE-SV40 - GCH2

    Plasmid
    #230815
    Purpose
    AAV cloning vector for CRE dependent expression under the CAG promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230815-plasmid-map-sequence-id-466637

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  14. AAV-GFP/Cre

    Plasmid
    #49056
    Purpose
    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.
    Depositor
    Fred Gage
    Article
    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99
    Insert
    Cre
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    EGFP, Myc, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49056-plasmid-map-sequence-id-182646

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  16. LeGO-iBSD-5LO-WT

    Plasmid
    #182741
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182741-plasmid-map-sequence-id-360423

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  18. Lenti-pNFkB::Cre-mRuby3

    Plasmid
    #164137
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    NFkB promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NFkB synthetic promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164137-plasmid-map-sequence-id-323778

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  19. hGFAP-Cre

    Plasmid
    #40591
    Purpose
    Mouse astrocyte expression of Cre
    Depositor
    Albee Messing
    Article
    Zhuo et al Genesis. 2001 Oct;31(2):85-94.
    Insert
    Cre recombinase (GFAP Phage, Mouse, Human)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Mammalian
    Mutation
    Construct contains a nuclear-targeted Cre recombi…
    Promoter
    hGFAP promoter
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40591-plasmid-map-sequence-id-302078

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  20. Lenti-pHSE::Cre-mRuby3

    Plasmid
    #164136
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Insert
    HSE promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HSE synthetic promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    164136-plasmid-map-sequence-id-323779

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  23. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229814-plasmid-map-sequence-id-460474

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  24. pGECPL.BC10.MG

    Plasmid
    #223822
    Purpose
    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.
    Depositor
    Dawid Nowak
    Article
    Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/
    Insert
    Ef1a-Driven Cre-P2A-FLuc
    Use
    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    223822-plasmid-map-sequence-id-443651

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  26. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  28. p221-RV-dlx-dio-Tdtomato

    Plasmid
    #185697
    Purpose
    Retroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoter
    Depositor
    Lynette Lim
    Insert
    membrane Tdtomato
    Use
    Cre/Lox and Retroviral
    Tags
    Gap43 palmitoylation
    Expression
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185697-plasmid-map-sequence-id-366336

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  29. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid
    #121546
    Purpose
    Super-bright fluorescence protein for tracking and visualization
    Depositor
    Matthias Prigge
    Insert
    cre-dependent mNeon
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121546-plasmid-map-sequence-id-308125

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  30. GFAP-Flex-MCS-WPRE-SV40 - GCH32

    Plasmid
    #230830
    Purpose
    AAV cloning vector for CRE dependent expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230830-plasmid-map-sequence-id-466634

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  31. TRE-Flex-MCS-WPRE-SV40 - GCH26

    Plasmid
    #230827
    Purpose
    AAV cloning vector for CRE dependent expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    230827-plasmid-map-sequence-id-466624

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  32. CaMKII-Flex-MCS-WPRE-SV40 - GCH20

    Plasmid
    #230824
    Purpose
    AAV cloning vector for CRE dependent expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230824-plasmid-map-sequence-id-459118

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  33. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid
    #230821
    Purpose
    AAV cloning vector for CRE dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230821-plasmid-map-sequence-id-457139

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  34. Syn-FLEX-MCS-WPRE-SV40 - GCH8

    Plasmid
    #230818
    Purpose
    AAV cloning vector for CRE dependent expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230818-plasmid-map-sequence-id-457133

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  36. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

    Plasmid
    #121542
    Purpose
    Chronos with red nucleus-targeted fluorescence for excitatory neurons
    Depositor
    Matthias Prigge
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CamKII
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121542-plasmid-map-sequence-id-308124

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  37. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  38. pAAV-synP-FLEX-TVA66T-EGFP

    Plasmid
    #64096
    Purpose
    Expresses TVA66T-EGFP under the synapsin promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    synapsin
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64096-plasmid-map-sequence-id-107236

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  39. pAAV-CAG-FLEX-TVA66T-EGFP

    Plasmid
    #64091
    Purpose
    Expresses TVA66T-EGFP under the CAG promotor
    Depositor
    Ian Wickersham
    Insert
    TVA66T-EGFP
    Use
    AAV and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glutamic acid 66 to Threonine
    Promoter
    CAG
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    64091-plasmid-map-sequence-id-107230

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  40. pAAV-SEO-CaMKII-EGFP

    Plasmid
    #177018
    Purpose
    For expression of EGFP in a single-floxed, excisable open reading frame (cre-off), under control of the CaMKIIa promoter
    Depositor
    Peter Jonas
    Insert
    EGFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177018-plasmid-map-sequence-id-353742

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  41. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Gary Yellen
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86920-plasmid-map-sequence-id-172992

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  42. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  43. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

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  44. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109059-plasmid-map-sequence-id-209551

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  45. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109058-plasmid-map-sequence-id-209548

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  46. pAAV-phSyn-flex-SypHaloTag

    Plasmid
    #172280
    Purpose
    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.
    Depositor
    Danny Winder
    Insert
    synaptophysin-HaloTag
    Use
    AAV and Cre/Lox
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    rat synapsin
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    172280-plasmid-map-sequence-id-337805

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  47. pDD289

    Plasmid
    #137018
    Purpose
    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    GFP-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137018-plasmid-map-sequence-id-291422

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  48. pBCSK_LEU2_II

    Plasmid
    #109061
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109061-plasmid-map-sequence-id-209544

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  49. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  50. pAAV-EF1a-iCreV

    Plasmid
    #140135
    Purpose
    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Has Service
    AAV PHP.eB and AAV1
    Insert
    iCreV
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140135-plasmid-map-sequence-id-292208

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Showing: 851 - 900 of 2919 results