We narrowed to 2,351 results for: hal.2
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Plasmid#117558PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtADH gene gene in A. thaliana with 1 mismatch at PAMDepositorInsertPromoter_AtU6-26 + sgRNA_atADH1{At1G77120) _1mismatch in PAM A. thaliana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0109
Plasmid#117571PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62590} A. thaliana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0098
Plasmid#117570PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62600} A. thaliana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-V5-APEX2 (in pLEX_307)
Plasmid#214921Purposeconstitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylationDepositorInsertSRRD (SRRD Human)
UseLentiviralTagsV5-APEX2ExpressionMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC32 [TU#839]
Plasmid#16079DepositorInsertmec-2 intron 9
UseTagsYFPExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDV18 [punc-47::SNG-1::CRY2olig(535)]
Plasmid#197598PurposeExpression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV12 [psng-1::SNG-1::CRY2olig(535)]
Plasmid#197596PurposePan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV06 [punc-17::SNG-1::CRY2(535)]
Plasmid#197597PurposeExpression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-DmNot3_677-844_P
Plasmid#147185PurposeBacterial Expression of DmNot3_677-844DepositorInsertDmNot3_677-844 (Not3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmNot3_677-844_P
Plasmid#147190PurposeBacterial Expression of DmNot3_677-844DepositorInsertDmNot3_677-844 (Not3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmNot3_677-844_P
Plasmid#147177PurposeBacterial Expression of DmNot3_677-844DepositorInsertDmNot3_677-844 (Not3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:AtFLS2
Plasmid#202186PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoterDepositorInsertFlagellin sensing 2 (FLS2 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-ADPG2
Plasmid#170723PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b-At 14-3-3 omega
Plasmid#44930DepositorInsert14-3-3 omega (GRF2 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-At 14-3-3 mu
Plasmid#44938DepositorInsert14-3-3 mu (GRF9 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pET15b-At 14-3-3 iota
Plasmid#44937DepositorInsert14-3-3 iota (GRF12 Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIR218-2_nt5G>U
Plasmid#174009PurposemiR218 nt5 point mutation in SLIT3DepositorUseTagsExpressionMammalianMutationNT5 G>U point mutation in SLIT3PromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRepCap6
Plasmid#110770PurposepRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.DepositorInsertAAV6 cap, AAV6 rep genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago1
Plasmid#10820DepositorInsertArgonaute 1 (AGO1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pIreSneo-FLAG/HA Ago3
Plasmid#10823DepositorInsertArgonaute 3 (AGO3 Human)
UseTagsFLAG and HAExpressionMammalianMutation1 silent mutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTPH418b
Plasmid#185725PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPH412
Plasmid#185724PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertecTadA(8e)-gp41-8N
UseCRISPRTagsExpressionBacterialMutationecTadA(8e) R26GPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPH405c
Plasmid#185726PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C) single PAM linker
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Hyg-omMx1
Plasmid#52420PurposeInducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 proteinDepositorInsertOncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
UseTagsExpressionMammalianMutationPromoterTRE2Available sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsFlagExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)
Plasmid#126026Purposeexpress RFP-tagged LARIAT variant for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHRolig-VHH(GFP)
UseTagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)
Plasmid#126025Purposeexpress RFP- and mCer-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70Available sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m1
Plasmid#204463PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR A127N mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m2
Plasmid#204464PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L128C mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_SulR(CDS)
Plasmid#186423Purposeprovides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)DepositorInsertSulR
UseSynthetic Biology; Cds (part b3-b4-b5), goldenbra…TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-mCherry
Plasmid#60032PurposeExpresses a fusion of CRY2PHR E490G with mCherryDepositorInsertCRY2PHR E490G (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationE490GPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-ABE8e
Plasmid#185671PurposeExpresses SpRY-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY
UseCRISPRTagsExpressionMammalianMutationSpRY D10APromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIRT1 Flag
Plasmid#13812PurposeMammalian expression of human SIRT1 with flag tagDepositorInsertSIRT1 (SIRT1 Human)
UseTagsflagExpressionMammalianMutationN-terminus truncatedPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA Null
Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2094_CRY2olig-miRFP
Plasmid#215114PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cellsDepositorInsertCRY2olig-miRFP (CRY2 Mustard Weed)
UseTagsmiRFPExpressionMammalianMutationE490GPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
UseTagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated optoDroplet
Plasmid#111507PurposeFor membrane localized optoDropletsDepositorInsertCry2 (CRY2 Mustard Weed)
UseLentiviralTagsFUS, Fusion Red, and Myristoylation Sequence (MYR…ExpressionMutationPromoterSFFVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m3
Plasmid#204465PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127S mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m4
Plasmid#204466PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127T mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m5
Plasmid#204467PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125F-A127G-S129N mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m6
Plasmid#204468PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-S129N-L130V mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m7
Plasmid#204469PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationLasR L125W-A127T-L128M mutationPromoterIn an operon and PLasAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_EGFP_KCNQ2 var4
Plasmid#173154PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_CyOFP1_KCNQ2 var4
Plasmid#173155PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-CyOFP1ExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only