We narrowed to 9,586 results for: Coli
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Plasmid#63824PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-31
Plasmid#63823PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-29
Plasmid#63822PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-21
Plasmid#63821PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-18
Plasmid#63819PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-8
Plasmid#63817PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Gm1
Plasmid#53528PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
TagsHis Tag and ThioredoxinExpressionBacterialPromoterT7Available SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
Tags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available SinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNH094
Plasmid#42167DepositorInsertmTFP1-RT-::[(G4S)3]::C-TAP Tag
Available SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNH090
Plasmid#42166DepositorInsertmTFP1::[(G4S)3]::C-TAP Tag
Available SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBB XIAP W310A/H467A
Plasmid#25682DepositorAvailable SinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEBB XIAP W310A
Plasmid#25677DepositorAvailable SinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_11
Plasmid#60582Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_11
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA
Plasmid#225079PurposeExpresses precursor peptide ChrA in E.coliDepositorInsertChrA
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CoiA1_CoiB
Plasmid#208761PurposePrecursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.DepositorInsertsCoiA1
CoiB
TagsHisx6 Tag and SUMOExpressionBacterialMutationChanged Ser 20 to Asn, Changed Arg 84 to Lys, botā¦PromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
TagsN-terminal His tag with thrombin cleavage siteExpressionBacterialAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I DCARDS
Plasmid#210828Purposeprotein expression in ecoliDepositorInserthRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
ExpressionBacterialMutationNAAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP-Cas9
Plasmid#62731PurposeExpresses SP-Cas9 protein in bacterial cellsDepositorInsertSP-CAS9
UseCRISPRTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hisMBP-AsCpf1-EC
Plasmid#79007PurposeExpressing his-MBP tagged AsCpf1 (E.coli codon optimized)DepositorInsertAsCpf1
Tags6xHis-MBP-NLS and NLS-HAExpressionBacterialPromotertac promoterAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-his-LbCpf1-EC
Plasmid#79008PurposeBacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)DepositorInsertLbCpf1
TagsMBP-6xHis-NLSExpressionBacterialPromotertac promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
Tags6xHis tagExpressionBacterialPromoterT7lacAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBB HA cIAP1
Plasmid#38232DepositorAvailable SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet3.0
Plasmid#174080PurposeTetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertTet3.0 tRNA synthetase and tRNA
ExpressionBacterialMutationL270G, Y271L, N311G, C313AAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
ExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST-2K
Plasmid#44564Purposefor ectopic constitutive expression of two mycobacterial proteins in one vectorDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_BauA
Plasmid#128952Purposeexpresses BauA in E.coliDepositorInsertferric acinetobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-66)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11d-hUba2
Plasmid#53136PurposeE.coli expression of full length untagged human Uba2 (SAE2)DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.colā¦ExpressionMammalianPromoterEF1aAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCOLA banana sensor sfGFP
Plasmid#107367PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNADepositorInsertbanana-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
TagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
PromoterLacUV5 and Trc promoterAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL1 BSMT1
Plasmid#106287PurposeExpresses BSMT1 in Ecoli off a T7 promoterDepositorInsertBSMT1
ExpressionBacterialPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30 GLTP
Plasmid#170733PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
Tags6x HisExpressionBacterialAvailable SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCas13 Twin-SUMO
Plasmid#199753PurposeExpresses codon optimized miniature mCas13 for protein expression in E.coliDepositorInsertmCas13
TagsSUMOExpressionBacterialPromoterLacIAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2+Nterm Spe1 +Cterm Age1 site
Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 77-465
Plasmid#45970DepositorInsertParkin 77-465 (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Deleā¦PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only