Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

174,739 results

Showing: 7281 - 7320 of 174739 results

  6. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

    Enlarge

  14. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

    Enlarge

  18. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

    Enlarge

  21. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid
    #85225
    Purpose
    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    John Naughton
    Inserts
    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    Use
    AAV and Cre/Lox
    Promoter
    hSyn
    Available Since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85225-plasmid-map-sequence-id-170787

    Enlarge

  22. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

    Enlarge

  24. pZ8-Ptac

    Plasmid
    #74064
    Purpose
    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74064-plasmid-map-sequence-id-138074

    Enlarge

  25. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

    Enlarge

  26. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

    Enlarge

  31. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Article
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Promoter
    mycobacterial promoter Psmyc
    Available Since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    70045-plasmid-map-sequence-id-488289

    Enlarge

  36. mCherry-Farnesyl-5

    Plasmid
    #55045
    Purpose
    Localization: Plasma Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55045-plasmid-map-sequence-id-314088

    Enlarge

  37. mCherry-Alpha-Actinin-19

    Plasmid
    #54975
    Purpose
    Localization: Alpha Actinin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    actinin alpha 1 (ACTN1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    54975-plasmid-map-sequence-id-152016

    Enlarge

Showing: 7281 - 7320 of 174739 results