163,606 results

1. pDG1-PHP.eB

   Plasmid

   #220799

   Purpose

   AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   E4, E2A, VA, AAV2 rep and PHP.eB cap genes
   

   Use

   AAV; Aav helper vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. His SUMO HNRNPA1 dHexa

   Plasmid

   #197013

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

   Insert

   HNRNPA1
   

   Use

   Tags

   6His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-BG4-NLS-mCer

   Plasmid

   #211448

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet1_CylLL_CylM

   Plasmid

   #208759

   Purpose

   Expresses modified cytolysin L (mCylLL) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CylLL
   CylLM
   

   Use

   Tags

   Hisx6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-Tet-On-shRNA-Control

   Plasmid

   #98398

   Purpose

   Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1749 (pDonor, R*MmeI)

   Plasmid

   #160733

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

   Plasmid

   #200904

   Purpose

   Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMYH9-WT-V5

   Plasmid

   #183512

   Purpose

   Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-VIVIT

   Plasmid

   #11106

   Depositor

   Anjana Rao

   Article

   Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

   Insert

   VIVIT
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBHSTC104

    Plasmid

    #124356

    Purpose

    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

    Depositor

    Hideo Iwai

    Article

    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

    Insert

    gp41-1 N-intein and C-intein (bicistronic)
    

    Use

    Tags

    CcdA-C (SFAD)

    Expression

    Bacterial

    Mutation

    Promoter

    STC

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-BetaActin-SecA5-YFP

    Plasmid

    #105664

    Purpose

    Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

    Depositor

    Qing Deng

    Article

    Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

    Insert

    Secreted Annexin5-EYFP (ANXA5 Human)
    

    Use

    Tol2-gateway

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Beta-Actin

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Luc

    Plasmid

    #45968

    Depositor

    Ralf Kuehn

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mAzurite-16xRhoBAST

    Plasmid

    #196347

    Purpose

    Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    mAzurite-16xRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-PIK3CA-H1047R

    Plasmid

    #116500

    Purpose

    Lentiviral expression of PIK3CA H1047R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    H1047R

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDV06.2c_DART-VADAR_iRFP720-sensor

    Plasmid

    #200410

    Purpose

    pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor detecting iRFP720
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MECP2 in pENTR

    Plasmid

    #216592

    Purpose

    gateway cloning donor vector containing MECP2

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    MECP2 (MECP2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT_TO_eGFP_TIA1

    Plasmid

    #106094

    Purpose

    gene expression of EGFP-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW-GFP-TurboID-GOI

    Plasmid

    #209381

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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