165,971 results

1. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pvPE-V4-Puro

   Plasmid

   #240290

   Purpose

   Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene into mammalian cells for gene editing.

   Depositor

   Lusheng Huang

   Article

   Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

   Insert

   pvPE-V4-Puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-mCherry

   Plasmid

   #114472

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-FAST-p10

   Plasmid

   #89152

   Purpose

   Express p10-FAST in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   NBV p10-FAST
   

   Expression

   Mammalian

   Promoter

   CAG promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-EGFP-C1-HsXRN1_K

   Plasmid

   #146784

   Purpose

   Mammalian Expression of HsXRN1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1 (XRN1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-EGFP-RhoA-Q63L

    Plasmid

    #12968

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA constitutively active (RHOA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DIO-BACE-HA

    Plasmid

    #232353

    Purpose

    Beta-Secretase to be expressed (1:1) with either ATLAS protein.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    BACE (BACE1 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcmv3-IRG1-HA

    Plasmid

    #198181

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1)

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    HA tag is added to C-terminal of target protein

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-Pur

    Plasmid

    #60507

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAS-NanoLuc

    Plasmid

    #87696

    Purpose

    Gal4VP16 driven expression of NanoLuc

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NanoLuc
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK419 (GAL-OsTIR1(F74G))

    Plasmid

    #140656

    Purpose

    GAL-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74G)
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. DTA in pCDEST2

    Plasmid

    #223705

    Purpose

    Mammalian expression vector encoding diptheria toxin A

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    Diphtheria toxin A
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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