We narrowed to 17,849 results for: Mut
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Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_P298L
Plasmid#104468Purposeexpress MBP-tagged full length hnRNPA2 P298LDepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EglN2 H358A-pBabe-puro
Plasmid#22705DepositorInsertEgg laying Nine - 2 (EglN2) (EGLN2 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationmutated cDNA: point mutation in codon 358 changi…PromoterAvailable SinceDec. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594V
Plasmid#131715PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMay 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-DEUT RUVBL1 Delete
Plasmid#159144PurposeExpression and protien purification of mutated RUVBL1DepositorInsertRUVBL1 (RUVBL1 Human)
UseTagsExpressionBacterialMutationamino acid 1-463, Glu134-Glu237 mutated to GPPGPromoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TE)
Plasmid#61522PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK3
Plasmid#98622PurposeGateway entry TOPO TA cloning vector for ELK3DepositorAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianMutationPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-V600K
Plasmid#131720PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceDec. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta2-29
Plasmid#124923PurposeFor expression of a.a.2-29 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSF-R(mA)-luc
Plasmid#12421DepositorInsertM-CSF receptor promoter fragment (CSF1R Human)
UseLuciferaseTagsExpressionMutationC/EBP binding site mutation (created a KpnI site …PromoterAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM45_WT
Plasmid#82892PurposeGateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81A
Plasmid#171248PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Ala (AR-W741C-S81A)PromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-W741C-S81D
Plasmid#171249PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)DepositorInsert3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Trp741Cys-Ser81Asp (AR-W741C-S81D)PromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AR-R405S
Plasmid#126051PurposeExpresses human androgen receptor (R405S) in mammalian cellsDepositorAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Mouse, Human)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
3159_pETcon-SARS-CoV-2-RBD-L452R-T478K
Plasmid#184407Purposeyeast surface display of the SARS-CoV-2 Delta variant RBDDepositorInsertSARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationL452R+T478KPromoterAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELF1
Plasmid#98627PurposeGateway entry TOPO TA cloning vector ELF1DepositorAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594E
Plasmid#131714PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-PIK3CA E545K
Plasmid#180032PurposeEntry vector of PIK3CA E545K for gateway cloning.DepositorInsertPIK3CA E545K (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationE545KPromoterAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Double
Plasmid#100729PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motif and …PromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500
Plasmid#92225PurposeExpression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue Y35 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Chicken ArcLight S174
Plasmid#53567PurposeGenetically encoded voltage sensor ArcLight with improved kineticsDepositorInsertChicken ArcLight-S174
UseTagsExpressionMammalianMutationGg-VSP contains an R153Q mutation and an amino ac…PromoterCMVAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (S610L)
Plasmid#31800DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged serine 610 to leucinePromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (E870K)
Plasmid#31802DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glutamic acid 870 to lysinePromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEE
Plasmid#159746PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T,RbsR-T]
Plasmid#60773PurposeContains PIq driving expression GalS-T, and PIq driving expression of RbsR-T.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with GalS LB…PromoterAvailable SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RVR
Plasmid#108863PurposeFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutationsDepositorInsertFnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R, K629R and N633R, Cpf1 is rice codon optimi…PromoterAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G466V
Plasmid#131722PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-WDR12-S127F
Plasmid#116707PurposeLentiviral expression of WDR12 S127FDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (E767K)
Plasmid#31801DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glutamic acid 767 to lysinePromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (G561E)
Plasmid#31799DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glycine 561 to glutamic acidPromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374S300/302A
Plasmid#62533PurposeN-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanineDepositorInserttruncated human Drosha mutant (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and changed serines 300…PromoterAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*mqg*
Plasmid#21174DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationFirst point mutation at nucleotide 5114 (numberin…PromoterAvailable SinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ROR1-T69M
Plasmid#116675PurposeLentiviral expression of ROR1 T69MDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-zeo: alpha6mGFP
Plasmid#35611Purposemammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tagDepositorInsertnAChr alpha6mEGFP (Chrna6 Mouse)
UseTagsmEGFP inserted after A405 with AGA linker on eith…ExpressionMammalianMutationPromoterCMVAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL6 sgRNA(MS2)-MS2-hA3a D131E
Plasmid#112132Purposeempty sgRNA cloning vector with MS2-humanA3a D131EDepositorInserthuman Apobec3a D131E mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131E mutant in hA3aPromoterhU6,CBhAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Y215A
Plasmid#100727PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform Y215A mutation (Y815A in full length)PromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-K651E
Plasmid#58685PurposeExpresses LGP2 with a mutation in the CTD (K651E) in mammalian cellsDepositorInsertLGP2-K651E (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationmutation to CTD, K651EPromoterCMVAvailable SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T,TreR-T]
Plasmid#60772PurposeContains PIq driving expression FruR-T, and PIq driving expression of TreR-T.DepositorInsertsFruR-T
TreR-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. TAN DBD with FruR LB…PromoterAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only