164,304 results

1. qTAG-AAVS1-Ef1a-Puro-mScarlet

   Plasmid

   #227274

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_dCas7-11

   Plasmid

   #197605

   Purpose

   Expresses inactive Cas7-11 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   dCas7-11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFosCreER

   Plasmid

   #46388

   Depositor

   Liqun Luo

   Article

   Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

   Inserts

   Fos (Fos Mouse)
   CreERT2
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-F-AF9

   Plasmid

   #49428

   Purpose

   Mammalian expression of Flag-tagged human AF9

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   AF-9 (MLLT3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK445 (BSD-P2A-BromoTag)

   Plasmid

   #214375

   Purpose

   BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-c-Met

   Plasmid

   #31786

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti-CAG-IKZF3-FLAG-IRES-GFP

   Plasmid

   #107388

   Purpose

   Lentiviral expression of IKZF3-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC34A2

   Plasmid

   #132088

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC34A2 (SLC34A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-13Myc-kanMX6

    Plasmid

    #39294

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Halo-SspB-PPM1H

    Plasmid

    #232870

    Purpose

    Expresses SspB-PPM1H in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    Halo-SspB-PPM1H (PPM1H )
    

    Use

    Tags

    Halo-SspB

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-SENP5

    Plasmid

    #34555

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP5 (SENP5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pACEBac1-PARP1-Flag-His-WT

    Plasmid

    #111572

    Purpose

    Expresses full length PARP1 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB6 (CK2alpha')

    Plasmid

    #27084

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' (CSNK2A2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM446 (Flag-DAS+4))

    Plasmid

    #108321

    Purpose

    Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Flag-DAS+4
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. CL20_mEGFP_KMT2A_MLLT10

    Plasmid

    #205843

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_MLLT10 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_MLLT10 (KMT2A, MLLT10 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNA_Cloning Vector BbsI

    Plasmid

    #128433

    Purpose

    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing

    Depositor

    Izuho Hatada

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 5'-myr-dEGFP-3'

    Plasmid

    #16075

    Depositor

    Erin Schuman

    Article

    Aakalu et al Neuron. 2001 May . 30(2):489-502.

    Insert

    Protein synthesis reporter (Camk2a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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