-
Plasmid#58801Purposeis a lentiviral vector coding the shRNA si1 (sigfp) and the gene of resistance to puromycin.DepositorInsertsi1
UseLentiviralTagsExpressionMutationPromoterHuman H1Available sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-miRFP670nano-P2A-EGFP
Plasmid#198064PurposeThe lentiviral vector for miRFP670nano and EGFPDepositorInsertmiRFP670nano
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.dTomato.miR30n.PGK.sfGFP.P2A.Tet3G
Plasmid#169282PurposeDoxycycline-inducible overexpression of miRNA or shRNA with dTomato as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.pre
Plasmid#169276PurposeConstitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFkB-TA-LUC-UBC-GFP-W
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-P2A-GFP-DOX-N
Plasmid#229407PurposeLentiviral or overpexression of cDNADepositorInsertMKI-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-6507-P2A-EGFP
Plasmid#197367PurposeThe lentiviral vector for NIR-GECO2(I426C) and EGFPDepositorInsertNIR-GECO2(I426C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationI426C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
Plasmid#44597DepositorInsertEF1a-GFP:2A:CHER(HA)
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2528-3971)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-XOPS-tdTOM-MOPS-GFP
Plasmid#44347DepositorInsertsXOPS-tdTOM
MOPS-GFP
UseLentiviralTagsExpressionMutationPromoterXenopus Opsin (XOPS) (pos 3131-4495) and mouse op…Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-mIRBP156-CHER-RK-GFP-WPRE
Plasmid#44593DepositorInsertsIRBP156-CHER
RK-GFP
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 3723-4019) and murine…Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-mIRBP156-CHER-WPRE
Plasmid#44351DepositorInsertsRK-GFP
mIRBP156-CHER
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2554-2850) and mouse …Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-MUT-P2A-GFP
Plasmid#229406PurposeLentiviral or overpexression of cDNADepositorInsertMKI-MUT-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationinteraction mutant L109G; L143G (Control mutant)PromoterEFSAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-6504-P2A-EGFP
Plasmid#197368PurposeThe lentiviral vector for NIR-GECOHI and EGFPDepositorInsertNIR-GECO2(C18S/I426C)
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationC18S/I426C mutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAExpressionMutationgRNA sequence: TACGTTCTCTATCACTGATPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUCas9GFP
Plasmid#85555PurposeExpresses Cas9 with green fluorescent reporterDepositorInserthumanised S.pyogenes Cas9
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUBCAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo
Plasmid#128453Purposelentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance geneDepositorInsertEf1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo
Plasmid#128455Purposelentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_8.4_Ex_CAMKIIA-EGFP-V5-SV40-Zeo
Plasmid#128464Purposelentiviral expression vector with CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertCAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo
Plasmid#128454Purposelentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1_186
Plasmid#159091Purposetargeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
FSW_SUN1_GFP
Plasmid#205985PurposeExpresses hSyn1-driven, GFP-tagged SUN1 to label neuronal nucleiDepositorInsertSUN1 (Sun1 Mouse)
UseLentiviralTagsSuperfolder GFPExpressionMutationPromoterhSyn1Available sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->sGFP-P2A-BFP
Plasmid#162234PurposeLentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cellsDepositorInsertEGFP and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->sGFP-P2A-mCherry
Plasmid#162232PurposeLentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cellsDepositorInsertEGFP and mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#5
Plasmid#176337PurposeThe synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#5
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->GssGFP-P2A-NLSIFP
Plasmid#162235PurposeLentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cellsDepositorInsertGFP - IFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#13
Plasmid#176338PurposeThe synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#13
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPAdGO2-PGK-Neo
Plasmid#136899PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPAdGO2
UseLentiviralTags2xNLSExpressionMutationTGG>TAGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GFP
Plasmid#175757PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPAdGO-PGK-Neo
Plasmid#136898PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPAdGO
UseLentiviralTags2xNLSExpressionMutationTGG>TGAPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-K40R
Plasmid#64059PurposeEGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO-PGK-Neo
Plasmid#136890PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-GFP-mSox2
Plasmid#206374PurposeExpresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSox2 (Sox2 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D
Plasmid#32460DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFLAGExpressionMutationS129D, S133D, S137DPromoterhuman PGKAvailable sinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLARRYv2-EGFP-T2A-Flpo
Plasmid#233212PurposeCloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selectionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Short_V5-GFP
Plasmid#221497PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-MTS-EGFP
Plasmid#190270PurposeLentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cellsDepositorInsertMTS-EGFP
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-TurboID-NES_pLX304
Plasmid#202019Purposeexpresses GFP-TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-GFP-TurboID-NES
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1_Short_V5-GFP
Plasmid#221499PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterUBCAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniCMV eGFP hPGK BSD
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP-HRP-TM_pLX304
Plasmid#202021Purposeexpresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vectorDepositorInsertFlag-GFP-HRP-TM
UseLentiviralTagsFlag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only