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We narrowed to 2,351 results for: hal.2

Showing: 901 - 950 of 2351 results

  1. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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  3. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

    Plasmid
    #214950
    Purpose
    Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.
    Depositor
    Dan Mulvihill
    Article
    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1
    Inserts
    VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
    VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
    Use
    Tags
    6xHis, Amino half of venus BIFC fragment, Carboxy…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    214950-plasmid-map-sequence-id-439535

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  4. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid
    #170725
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Citrine tag (714bp)
    Expression
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170725-plasmid-map-sequence-id-345738

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  6. CRY2-OCRL

    Plasmid
    #79566
    Purpose
    expression of CRY2PHR fused to inositol 5-phosphatase domain of OCRL
    Depositor
    Olof Idevall-Hagren
    Insert
    OCRL (OCRL Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    aa 234_539
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147260-plasmid-map-sequence-id-289707

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  11. TRE-mClover3-TDP-43ΔNLS

    Plasmid
    #214917
    Purpose
    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta
    Depositor
    Ophir Shalem
    Article
    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d
    Insert
    TDP-43ΔNLS (TARDBP Human)
    Use
    Lentiviral
    Tags
    mClover3
    Expression
    Mutation
    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214917-plasmid-map-sequence-id-424953

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  13. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

    Plasmid
    #197599
    Purpose
    Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive
    Depositor
    Alexander Gottschalk
    Article
    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:
    Insert
    Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    D387A, E490G
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197599-plasmid-map-sequence-id-410713

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  22. CMV/N3IC-HA

    Plasmid
    #47618
    Purpose
    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain
    Depositor
    Urban Lendahl
    Article
    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.
    Insert
    Notch3 Intracellular domain (Notch3 Mouse)
    Use
    In vitro transcription and translation
    Tags
    HA
    Expression
    Mammalian
    Mutation
    codons 1664 (Met) to 2318 (Ala)
    Promoter
    CMV
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pMZ103-rbs1-m8

    Plasmid
    #204470
    Purpose
    This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58
    Inserts
    quorum sensing regulator LasR (lasR )
    eYFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    LasR L128F-S129W mutation
    Promoter
    In an operon and PLas
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    204470-plasmid-map-sequence-id-403702

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  25. gRNA NIA TLS2

    Plasmid
    #196981
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    AtNIA1 gRNAs fused to TLS2 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196981-plasmid-map-sequence-id-390454

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  26. gRNA NIA TLS1

    Plasmid
    #196980
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    AtNIA1 gRNAs fused to TLS1 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196980-plasmid-map-sequence-id-390456

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  36. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

    Plasmid
    #147279
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147279-plasmid-map-sequence-id-355936

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  37. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

    Plasmid
    #147264
    Purpose
    Insect Expression of DmNot3-mut2-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-mut2-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147264-plasmid-map-sequence-id-289711

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  38. pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q

    Plasmid
    #147261
    Purpose
    Insect Expression of DmNot3-F764A-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-F764A-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147261-plasmid-map-sequence-id-289708

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  39. pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q

    Plasmid
    #147263
    Purpose
    Insect Expression of DmNot3-mut1-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-mut1-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147263-plasmid-map-sequence-id-289710

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  40. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid
    #147262
    Purpose
    Insect Expression of DmNot3-F818A-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-F818A-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147262-plasmid-map-sequence-id-355929

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  41. pOPINO_C5

    Plasmid
    #171925
    Purpose
    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C5
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171925-plasmid-map-sequence-id-338635

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  42. pOPINO_C1

    Plasmid
    #171924
    Purpose
    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_C1
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171924-plasmid-map-sequence-id-338631

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  43. T7-VEE-IRES-Puro

    Plasmid
    #58970
    Purpose
    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Type
    Empty backbone
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58970-plasmid-map-sequence-id-91400

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Showing: 901 - 950 of 2351 results