We narrowed to 16,514 results for: puromycin

1. PAX6 donor plasmid

   Plasmid

   #105239

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
   

   Use

   TALEN

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-Stat5a1*6

   Plasmid

   #130668

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. E[beta]P

   Plasmid

   #24313

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMLV-CAG-TVA-IRES-Puro

   Plasmid

   #172366

   Purpose

   Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + Puro
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPyCAG-mKate2-3xNLS-IRES-Pac

   Plasmid

   #183618

   Purpose

   Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

   Insert

   mKate2-3xNLS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. betaRBPIG

   Plasmid

   #16422

   Depositor

   Juergen Hescheler

   Article

   Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

   Insert

   loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP
   

   Expression

   Mammalian

   Available Since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pUREF-EX

   Plasmid

   #199331

   Purpose

   A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

   Depositor

   Rikiro Fukunaga

   Article

   Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-sgTel

   Plasmid

   #122659

   Purpose

   Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPL5618_pUG_MtxR

   Plasmid

   #60929

   Purpose

   PCR template vector for generating methotrexate resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Dihydrofolate reductase (DHFR Human)
   

   Use

   Loxp flanked deletion cassette template

   Mutation

   L22F F31S

   Promoter

   TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-gRNA-puro

    Plasmid

    #180426

    Purpose

    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    gRNAscr
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. attB53-Pac-attB53

    Plasmid

    #183610

    Purpose

    RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Pac
    

    Use

    Rmce

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TOPO PuroR

    Plasmid

    #68447

    Purpose

    TOPO construct expressing PuroR for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-puro-eGFP-HEC1-9A

    Plasmid

    #114023

    Purpose

    Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
    

    Expression

    Mammalian

    Mutation

    Hec1 - 9A

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3PA-Puro-iTk

    Plasmid

    #109067

    Purpose

    inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

    Depositor

    Eric Lander

    Article

    Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS1021-Tier1-PhCMV-PuroR

    Plasmid

    #169492

    Purpose

    Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven puromycin N-acetyl-transferase
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-sg-CMV152

    Plasmid

    #179916

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-CMV51

    Plasmid

    #179915

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-sg-CMV162

    Plasmid

    #179917

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-sg-mGreenLantern332

    Plasmid

    #179914

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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