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161,641 results

Showing: 3781 - 3800 of 161641 results

  3. miniCRISPRoff-v1

    Plasmid
    #197030
    Purpose
    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized miniCRISRPoff-v1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197030-plasmid-map-sequence-id-388972

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  5. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  6. pET24a-VHH-mCherry

    Plasmid
    #109421
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109421-plasmid-map-sequence-id-211587

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  11. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  14. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  16. pJL 89

    Plasmid
    #98558
    Purpose
    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Enh 35S
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98558-plasmid-map-sequence-id-192654

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  17. tdTomato-ZO1-C-14

    Plasmid
    #58147
    Purpose
    Localization: Tight Junctions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid
    #26429
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26429-plasmid-map-sequence-id-354259

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Showing: 3781 - 3800 of 161641 results