159,237 results

1. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

   Plasmid

   #171019

   Purpose

   Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   LargeBit-SNAPtag- Beta-actin
   

   Use

   Lentiviral and Luciferase

   Tags

   Beta actin and LargeBiT

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIVh

   Plasmid

   #81091

   Purpose

   Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a - Ec-ClpX

   Plasmid

   #111516

   Purpose

   Expresses E. coli ClpX with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   ClpX
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3_UnaG-Flag-Sec61B

   Plasmid

   #83413

   Purpose

   Expressed N-term tagged UnaG on Sec61B

   Depositor

   Hyun-Woo Rhee

   Article

   Park et al ACS Chem Biol. 2016 May 27.

   Insert

   UnaG-Sec61B (SEC61B Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST/N1-hBIN1-GFP

   Plasmid

   #27305

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ136

   Plasmid

   #162665

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-puro

   Plasmid

   #39481

   Purpose

   Tetracycline inducible 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5 and 6XHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS42H_PTEF1_dCas9_TCYC1

   Plasmid

   #177155

   Purpose

   Expresses dCas9 in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Insert

   Nuclease-null Cas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2424_Nme2.Smu-ABE8e-i8

   Plasmid

   #201522

   Purpose

   Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2.Smu-ABE8e-i8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPCX-VEcadTS

    Plasmid

    #45848

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin Tension Sensor (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Use

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-HA-OTUD5

    Plasmid

    #22610

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Use

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. IKK2-EGFP

    Plasmid

    #111195

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    IKKbeta (IKBKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p5E-4xnrUAS (L4-R1)

    Plasmid

    #61372

    Purpose

    5' Gateway Entry Clone Containing 4xnrUAS

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnrUAS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-H2B-tdTomato-SRT

    Plasmid

    #203393

    Purpose

    AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    H2B fused piggyBac tdTomato self-reporting transposon
    

    Use

    AAV

    Tags

    3X SV40 and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-ERBB2

    Plasmid

    #23888

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pJL1-sfGFP-DQNAT

    Plasmid

    #198210

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_DQNAT
    

    Use

    Tags

    6x His Tag and DQNAT glycosylation tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS-mCherry

    Plasmid

    #135457

    Purpose

    Mammalian mCherry reporter to read out FLARE and SPARK activation

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist GFP-Linker-VDAC1

    Plasmid

    #211735

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge