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We narrowed to 23,099 results for: crispr

Showing: 9451 - 9500 of 23099 results

  15. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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  17. pEHF-donor

    Plasmid
    #113817
    Purpose
    CRISPR donor plasmid to tag human transcription factor EHF with GFP
    Depositor
    Kevin White
    Insert
    EHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs3818514, rs11032804, hg38:11:34659087:G>A
    Promoter
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113817-plasmid-map-sequence-id-230183

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  18. pBCL6-donor

    Plasmid
    #112389
    Purpose
    CRISPR donor plasmid to tag human transcription factor BCL6 with GFP
    Depositor
    Kevin White
    Insert
    BCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112389-plasmid-map-sequence-id-229853

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  19. pSRF-donor

    Plasmid
    #112362
    Purpose
    CRISPR donor plasmid to tag human transcription factor SRF with GFP
    Depositor
    Kevin White
    Insert
    SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:6:43178470:C>A, hg38:6:43178514:G>A
    Promoter
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112362-plasmid-map-sequence-id-229685

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  20. pZNF766-donor

    Plasmid
    #112352
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF766 with GFP
    Depositor
    Kevin White
    Insert
    ZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:52290357:G>T, hg38:19:52290373:A>G,…
    Promoter
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112352-plasmid-map-sequence-id-229667

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  21. pVEZF1-donor

    Plasmid
    #112341
    Purpose
    CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP
    Depositor
    Kevin White
    Insert
    VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs999907002, hg38:17:57975308:A>T, hg38:17:579…
    Promoter
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112341-plasmid-map-sequence-id-229654

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  22. pELK3-donor

    Plasmid
    #112322
    Purpose
    CRISPR donor plasmid to tag human transcription factor ELK3 with GFP
    Depositor
    Kevin White
    Insert
    ELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:96266960:A>T, rs760335647, rs6538704
    Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112322-plasmid-map-sequence-id-228533

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  33. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  36. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  42. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  43. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

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  44. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

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  50. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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Showing: 9451 - 9500 of 23099 results