Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,106 results for: UTY

Clear keywords
Showing: 9651 - 9700 of 10106 results

  1. pXPR_220

    Plasmid
    #164560
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164560-plasmid-map-sequence-id-330240

    Enlarge

  8. Rescue myc-PCDHGC5 mRNA

    Plasmid
    #122229
    Purpose
    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.
    Insert
    PCDHGC5 (Pcdhgc5 Rat)
    Tags
    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…
    Expression
    Mammalian
    Mutation
    Silent mutations are in five consecutive codons …
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122229-plasmid-map-sequence-id-237759

    Enlarge

  9. ORAI1-YFP V107M-L273D-L276D

    Plasmid
    #114183
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M/L273D/L276D
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114183-plasmid-map-sequence-id-218685

    Enlarge

  17. pLenti PGK Puro GFP-GABARAPL1 G116

    Plasmid
    #123243
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acid 117. Stop codon after G116
    Promoter
    PGK
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123243-plasmid-map-sequence-id-244543

    Enlarge

  18. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

    Enlarge

  19. pLP-PIGA3 (pLP305)

    Plasmid
    #196992
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196992-plasmid-map-sequence-id-389805

    Enlarge

  20. pLenti PGK Puro GFP-GABARAPL1 G116A

    Plasmid
    #123245
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 116 to Alanine
    Promoter
    PGK
    Available Since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    123245-plasmid-map-sequence-id-245084

    Enlarge

  23. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

    Enlarge

  24. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid
    #208038
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208038-plasmid-map-sequence-id-410203

    Enlarge

  25. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available Since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

    Enlarge

  26. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

    Enlarge

  27. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Promoter
    EF-1a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

    Enlarge

  28. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

    Enlarge

  29. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid
    #236007
    Purpose
    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835Y substitution
    Promoter
    CMV
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236007-plasmid-map-sequence-id-469607

    Enlarge

  33. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

    Enlarge

  35. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid
    #218973
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Expression
    Mammalian
    Mutation
    mutant SRSF1 binding motif (AGTCTCCT)
    Promoter
    RSV LTR
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218973-plasmid-map-sequence-id-436111

    Enlarge

  38. pDule2-3-nitroTyrosine (A7)

    Plasmid
    #174079
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70T D158H I159A L162R
    Promoter
    GlnRS (constitutive)
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174079-plasmid-map-sequence-id-341130

    Enlarge

  40. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid
    #236008
    Purpose
    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835N substitution
    Promoter
    CMV
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236008-plasmid-map-sequence-id-469587

    Enlarge

  43. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

    Enlarge

  46. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Promoter
    RPBSA and TRE promoter
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

    Enlarge

  47. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

    Enlarge

  50. LPAR1-DuET

    Plasmid
    #213329
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR1 (LPAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213329-plasmid-map-sequence-id-430863

    Enlarge

Showing: 9651 - 9700 of 10106 results