163,606 results

1. pDEST/LIfeAct-mCherry-N1

   Plasmid

   #40908

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   LifeAct
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   No stop codon to create c-terminal fusion

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFJ151 - ttTi5605_MCS

   Plasmid

   #19330

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   ttTi5605 targeting region
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PE7-SB-NLS

   Plasmid

   #228870

   Purpose

   PE7 connected to MLH1-SB-NLS via 2A sequence

   Depositor

   Sangsu Bae

   Article

   Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

   Insert

   PE7-2A-MLH1-SB-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET22b-msfGFP-NPM1

   Plasmid

   #237670

   Purpose

   For expression of msfGFP-NPM1 in Ecoli

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-DDX4N-mCh-Cry2WT

   Plasmid

   #101225

   Purpose

   DDX4(1-236) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   DDX4(1-236)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_Pro-IL-33

   Plasmid

   #214342

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL33 (IL33 Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-smURFP

   Plasmid

   #80349

   Purpose

   Lentiviral transfer vector under the CMV promoter that expresses smURFP.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Insert

   smURFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SIRT2 Flag

   Plasmid

   #13813

   Purpose

   Mammalian expression of human SIRT2 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Transcript variant 2

   Promoter

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-HP1alpha

   Plasmid

   #179701

   Purpose

   visualization of centromere heterochromatin

   Depositor

   Kenji Sugimoto

   Article

   Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

   Insert

   heterochromatin protein alpha (CBX5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR OVA/p2a/mCherry-CaaX

    Plasmid

    #113030

    Purpose

    for lentivirus production of ovalbumin and mcherry-caax

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    OVA/p2a/mCherry-CaaX
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTR-345 pUC19-tNGFR-P2A-IL2RB N

    Plasmid

    #186064

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RB (IL2RB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGART-GFP

    Plasmid

    #99106

    Purpose

    Expresses human GART-GFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    GART (GART Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A65T, L68V, and A72S mutations in GFP gene; kanR/…

    Promoter

    CMV

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCF525-AcrIIA4_gb39_lenti

    Plasmid

    #138305

    Purpose

    Lenti expression of AcrIIA4

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3xFLAG-TYR

    Plasmid

    #32780

    Depositor

    Ruth Halaban

    Article

    Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pUCmini-iCAP-AAV.CAP-Mac

    Plasmid

    #200658

    Purpose

    Synthetic construct isolate AAV.CAP-Mac VP1 gene

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    Synthetic construct isolate AAV.CAP-Mac VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    7 amino acid insertion after Q588 of the AAV9 CAP…

    Promoter

    p41

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIGAhRC

    Plasmid

    #112510

    Purpose

    human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    HIF1B, AhR (ARNT, AHR Human, Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1,10

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tdTomato-C1

    Plasmid

    #54653

    Purpose

    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSAG1::CAS9-U6::sgUPRT

    Plasmid

    #54467

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    SAG1 5' UTR
    

    Use

    CRISPR; Toxoplasma expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

    Plasmid

    #218761

    Purpose

    Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF0219

    Plasmid

    #142563

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ADNP (ADNP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid

    #164514

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSPble

    Plasmid

    #63561

    Purpose

    Bleomycin drug selection marker

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Bleomycin drug selection marker
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGD-γb

    Plasmid

    #196327

    Purpose

    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    barley stripe mosaic virus γb
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-Ftractin-mCherry

    Plasmid

    #85131

    Purpose

    lentiviral expression of an F-actin live-cell marker (mCherry)

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYMA3_SidK_GFP

    Plasmid

    #184276

    Purpose

    Legionella pneumophila SidK (1-278) - GFP

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSFDuet-1_DNAseI

    Plasmid

    #182408

    Purpose

    Bacterial expression of DNaseI

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid

    #220611

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mApple-Golgi-7

    Plasmid

    #54907

    Purpose

    Localization: Golgi Complex, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

36. HA-cdh1

    Plasmid

    #11596

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdh1 (FZR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23119 and ptipA

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPM28 (eroGFP CEN/ARS URA3)

    Plasmid

    #20131

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    eroGFP (KAR2 jellyfish, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDA077

    Plasmid

    #131388

    Purpose

    L1RP expression

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHTN-HaloTag_DDB1:1-1140

    Plasmid

    #211006

    Purpose

    Mammalian expression of full-length human DDB1. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DDB1:M1-H1140 (DDB1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LeGO-iG2

    Plasmid

    #27341

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET19-TdTwt

    Plasmid

    #207466

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. GSK3 beta pGEX

    Plasmid

    #15898

    Depositor

    Jim Woodgett

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 1, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. plenti-CMV-mCherry-T2A-GFP

    Plasmid

    #109427

    Purpose

    A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. mNeonGreen-APEX3

    Plasmid

    #202590

    Purpose

    Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

    Depositor

    Reuben Harris

    Article

    Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

    Insert

    mNeonGreen-APEX3-V5 (APX1 )
    

    Use

    Retroviral

    Tags

    V5 epitope and mNeonGreen

    Expression

    Mammalian

    Mutation

    APEX2 L242A

    Promoter

    MESV LTR

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. CF-Inp(D281A)

    Plasmid

    #20156

    Depositor

    Tobias Meyer

    Article

    Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

    Insert

    CFP-FKBP-Inp54p (D281A) (INP54 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Stop codon at position 331. D281A mutation.

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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