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175,647 results

Showing: 9701 - 9750 of 175647 results

  2. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    90518-plasmid-map-sequence-id-288271

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  12. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

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  15. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86979-plasmid-map-sequence-id-514761

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  16. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  17. Lenti-LbCpf1-Blast

    Plasmid
    #84751
    Purpose
    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    LbCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84751-plasmid-map-sequence-id-164465

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  21. p5E--3mnx1

    Plasmid
    #74632
    Purpose
    Can be used to drive expression specifically in zebrafish motor neurons
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    -3mnx1
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    74632-plasmid-map-sequence-id-207910

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  22. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75024-plasmid-map-sequence-id-450679

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  26. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  29. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  35. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid
    #73147
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-NRX
    Use
    Lentiviral
    Tags
    2x HA tag and Flag tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73147-plasmid-map-sequence-id-134524

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  40. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

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  42. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75349-plasmid-map-sequence-id-266810

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Showing: 9701 - 9750 of 175647 results