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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 38,411 results for: ANT

Showing: 7841 - 7880 of 38411 results

  1. SUCNR1-DuET

    Plasmid
    #213381
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SUCNR1 (SUCNR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213381-plasmid-map-sequence-id-430736

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  2. S1PR4-DuET

    Plasmid
    #213375
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    S1PR4 (S1PR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213375-plasmid-map-sequence-id-430731

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  3. SSTR1-DuET

    Plasmid
    #213376
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR1 (SSTR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213376-plasmid-map-sequence-id-430732

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  4. PTGER1-DuET

    Plasmid
    #213370
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    PTGER1 (PTGER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213370-plasmid-map-sequence-id-430729

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  5. pGWB602-OEP7-mVenus

    Plasmid
    #213864
    Purpose
    Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do
    Insert
    chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213864-plasmid-map-sequence-id-425332

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  8. pGG-GTS-TurboID-GFP

    Plasmid
    #209405
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    GTS-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209405-plasmid-map-sequence-id-418457

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  9. pGG-mTP-TurboID-GFP

    Plasmid
    #209404
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    mTP-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209404-plasmid-map-sequence-id-418443

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  19. pN1mcherry-NKD2G2A

    Plasmid
    #174334
    Purpose
    encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    mutation of a.a 2 Gly to Ala
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174334-plasmid-map-sequence-id-349873

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  20. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Sonal Srikanth
    Article
    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174333-plasmid-map-sequence-id-349872

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  21. TMD18

    Plasmid
    #163673
    Purpose
    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163673-plasmid-map-sequence-id-322677

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  22. TMD19

    Plasmid
    #163674
    Purpose
    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163674-plasmid-map-sequence-id-322678

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  23. EGFP-acVHH (Y34W)-PB

    Plasmid
    #171072
    Purpose
    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34W
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171072-plasmid-map-sequence-id-338143

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  24. EGFP-acVHH (Y34F)-PB

    Plasmid
    #171071
    Purpose
    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34F
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171071-plasmid-map-sequence-id-338141

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  25. EGFP–acVHH (Y104F)–PB

    Plasmid
    #169683
    Purpose
    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104F
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169683-plasmid-map-sequence-id-338137

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  27. eNS2B47NS3 S135A

    Plasmid
    #155318
    Purpose
    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutant
    Depositor
    Dahai Luo
    Article
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    S135A
    Promoter
    M13
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155318-plasmid-map-sequence-id-311249

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  35. pVH003

    Plasmid
    #80397
    Purpose
    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Anti-scaffold - Venus YFP
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    Fused by GS linker to LZx domain and Venus has N-…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80397-plasmid-map-sequence-id-155890

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Showing: 7841 - 7880 of 38411 results