We narrowed to 16,979 results for: She

1. pMito-miRFP718nano

   Plasmid

   #195754

   Purpose

   Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   Human cytochrome C oxidase subunit VIII-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_HcRed

   Plasmid

   #25892

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   hcRed
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH2B-miRFP718nano

   Plasmid

   #195750

   Purpose

   Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   H2B-miRFP718nano
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRK5 CASTOR1-HA Cterm

   Plasmid

   #84487

   Purpose

   overexpression of CASTOR1 with HA tag

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pSR62

   Plasmid

   #125076

   Purpose

   Expression of ompR under PLtetO-1, output promoter PompF146

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   ompR (ompR )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTmpKmH207in

   Plasmid

   #176230

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTmpKmH207out

   Plasmid

   #176231

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTmpKmA1O4in

   Plasmid

   #176232

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTmpKmA1O4out

   Plasmid

   #176233

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDestTol2CG2--3.5ubb:plin2-tdtomato

    Plasmid

    #172534

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYFAC-CH2

    Plasmid

    #168978

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-SYN-eGFP-Kindlin 1

    Plasmid

    #203733

    Purpose

    AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    Fermt1 (Fermt1 Mouse)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYFAC-CH4

    Plasmid

    #168980

    Purpose

    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pv6_EGFP

    Plasmid

    #179392

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    EGFP
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OVA-miSFIT-17-T

    Plasmid

    #124677

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLHCX-Flag-mPKM1

    Plasmid

    #42511

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M1
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 PhLP1-myc-6xHis

    Plasmid

    #218176

    Purpose

    Expresses phosducin-like protein 1 in mammalian cells

    Depositor

    Barry Willardson

    Article

    Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

    Insert

    Phosducin like protein 1 (PDCL Human)
    

    Use

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSRalpha-MSV-mouse Cdk4wt-Tk-neo

    Plasmid

    #115525

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Matsushime et al Cell. 1992 Oct 16;71(2):323-34.

    Insert

    Mouse Cdk4 (Cdk4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

22. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF

    Plasmid

    #163317

    Purpose

    Encodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase

    Depositor

    Dawn Chandler

    Insert

    Nsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Dead Streptavidin-SpyTag

    Plasmid

    #59548

    Purpose

    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead Streptavidin-SpyTag
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    The construct lacks final K of SpyTag

    Promoter

    T7

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

25. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJFRC-20XUAS-IVS-PhiC31

    Plasmid

    #133565

    Purpose

    Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    PhiC31 Recombinase (int )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    20X UAS

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AICSDP-078:G3BP1-mEGFP

    Plasmid

    #193920

    Purpose

    Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

    Depositor

    Allen Institute for Cell Science

    Insert

    G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pBabe_puro_DEST_Flag_TNIK

    Plasmid

    #45276

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TNIK (TNIK Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTRY-YFP

    Plasmid

    #15302

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YFP
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Expression

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE hSPC-dsRed Express-W

    Plasmid

    #36449

    Depositor

    Darrell Kotton

    Article

    Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

    Inserts

    human SP-C promoter
    dsRed Express
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    N/A and human SP-C promoter

    Available since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. shLacZ

    Plasmid

    #42559

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shLacZ
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

    Plasmid

    #172535

    Purpose

    Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

    Depositor

    Richard White

    Article

    Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

    Insert

    -3.5ubb:plin2-tdtomato
    

    Use

    Tags

    Expression

    Mutation

    S80R, A81S, E144D, I148F and R415H mutations obse…

    Promoter

    zebrafish ubb

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRepair-MPP8_mAID-P2A-BFP

    Plasmid

    #175036

    Purpose

    Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    mAID-P2A-BFP
    

    Use

    Hdr donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FLAG-hIes6

    Plasmid

    #15356

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    hIes6 (INO80C Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GST-CHMP4A (pGEX2T(TEV))

    Plasmid

    #80630

    Purpose

    Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-222

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUBC_V5_MS2-mVenus

    Plasmid

    #107606

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    MS2-mVenus
    

    Use

    Synthetic Biology

    Tags

    V5 and mVenus

    Expression

    Mutation

    MS2: V75E/A81G

    Promoter

    human UBC

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pYFAC-CH3

    Plasmid

    #168979

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1-TAT-1-101-FLAG basic mutant

    Plasmid

    #35985

    Depositor

    Susana Valente

    Article

    Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

    Insert

    transactivating protein (tat )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    tat from HIV1, 101 aa; R to A mutations at aa 56,…

    Promoter

    Available since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GFP-GLT1b

    Plasmid

    #162516

    Purpose

    Rat GLT-1b cDNA N-terminally tagged with EGFP

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

44. pCMV-LOx-IRES-EGFP

    Plasmid

    #166877

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pNHEJ-RPG

    Plasmid

    #85931

    Purpose

    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

    Depositor

    Zhiying Zhang

    Article

    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

    Insert

    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    

    Use

    Dual-reporter surrogate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. c3GIC9n

    Plasmid

    #62192

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9 (D10A)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    D10A substitution (Cas9n)

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEPQD2KN0908

    Plasmid

    #177088

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19, CrDXS2, PaGPPS1, and CrGES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA flag-Pcbp2

    Plasmid

    #184028

    Purpose

    Codon optimized Pcbp2 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Pcbp2 (Pcbp2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pN1-CMV-L-sDarken

    Plasmid

    #184801

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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