We narrowed to 31,552 results for: Eng;

1. pJW1219

   Plasmid

   #61250

   Purpose

   CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-TO-MCP-3XBFPnls

   Plasmid

   #75383

   Purpose

   MCP-3XBFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   3XBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK1050

   Plasmid

   #209540

   Purpose

   BTK Type 1 homology arm for Golden Gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pBTK1051

   Plasmid

   #209541

   Purpose

   BTK Type 5 homology arm for Golden Gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pMEG_YLCas9_empty

   Plasmid

   #117826

   Purpose

   CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpTEF_hCas9_YlCyc1TT
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2lox-cAMPr R739A

   Plasmid

   #102618

   Purpose

   cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

   Depositor

   Justin Blau

   Article

   Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

   Insert

   cAMPr
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   R739A

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-TRE3G-tdTomato

   Plasmid

   #194644

   Purpose

   Expresses tdTomato under the control of TRE3G

   Depositor

   Jun-Hyeong Cho

   Article

   Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

   Insert

   TRE3G-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3s-Cas9HC

   Plasmid

   #43945

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA epitope and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA0.421

   Plasmid

   #203950

   Purpose

   Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNBU2_erm_P1T_DP-GH023

    Plasmid

    #90317

    Purpose

    Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGD425

    Plasmid

    #132381

    Purpose

    contains the sequence of mTurquoise2 for C-term fusions in the pGGD000 backbone (GreenGate)

    Depositor

    Rüdiger Simon

    Article

    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

    Insert

    linker-mTurquoise2
    

    Use

    Greengate entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIF4

    Plasmid

    #196069

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MoRP27 promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW1285

    Plasmid

    #61252

    Purpose

    CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting pha-1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG504-frFAST

    Plasmid

    #160055

    Purpose

    Expresses frFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

    Insert

    frFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BDS-2 3x WT (p53 binding site)

    Plasmid

    #16515

    Depositor

    Bert Vogelstein

    Article

    Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

    Insert

    p53 binding site (SFN Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-SpyCatcher-mi3

    Plasmid

    #112255

    Purpose

    Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    SpyCatcher-mi3
    

    Use

    Tags

    C-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pADS

    Plasmid

    #19040

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    codon-optimized synthetic amorphadiene synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pGEX Smad3

    Plasmid

    #12630

    Depositor

    Rik Derynck

    Article

    Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pENTR APP shRNA 2

    Plasmid

    #30135

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP shRNA 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pJMH143

    Plasmid

    #186646

    Purpose

    aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co-TaadA-PJ23117-eYFP.co
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pP2-P1(S')

    Plasmid

    #196334

    Purpose

    Plasmid expressing chimeric P2-P1(S') tail fiber

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    chimeric tail fiber P2-P1(S') and chaperone protein U'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P2 late promoter

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGP-FF

    Plasmid

    #13480

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Gal11P

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-Himar-dCas9

    Plasmid

    #137079

    Purpose

    Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDA010.EL222

    Plasmid

    #206801

    Purpose

    J23106.EL222

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    EL222
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    J23106

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-TO-PV-NES-DsRed

    Plasmid

    #16339

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-NES-DsRed (Pvalb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pHR_EF1a-->HA-mtLaG2

    Plasmid

    #162225

    Purpose

    Lentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody LaG2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGT-B1

    Plasmid

    #122579

    Purpose

    MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-Wnk1

    Plasmid

    #38780

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pcS-mCherry-C1C2

    Plasmid

    #178425

    Purpose

    Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Opto-hRET_317

    Plasmid

    #58747

    Purpose

    Expresses Opto-hRET (light-activated receptor tyrosine kinase)

    Depositor

    Harald Janovjak

    Article

    Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

    Insert

    Opto-hRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. C245

    Plasmid

    #174351

    Purpose

    Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    rpl3.L (rpl3.L Frog)
    

    Use

    In vitro transcription template

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-51b-HaloTag11

    Plasmid

    #175519

    Purpose

    Expression of HaloTag11 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag11
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIF3

    Plasmid

    #196068

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MoRP27 promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKM489-Snap

    Plasmid

    #108437

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with SNAP tag.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-SNAP tag
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLNC Wnt-5aHA

    Plasmid

    #18032

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5a (Wnt5a Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pCfB4170

    Plasmid

    #126750

    Purpose

    Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413

    Depositor

    Michael Jensen

    Article

    Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

    Insert

    yEGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTRX2_5xUAS

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-mRuby2

    Plasmid

    #89686

    Purpose

    pCAG promoter driving mRuby2

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDW1775

    Plasmid

    #13456

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    AcV5

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSARSF53-110

    Plasmid

    #61813

    Purpose

    TvoVMAN11 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    TvoVMAN11 intein
    

    Use

    Tags

    H6-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSABAD14-98

    Plasmid

    #61827

    Purpose

    TvoVMAdN11 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    TvoVMAdN11 intein
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge