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Plasmid#182006PurposeCMV overexpression of SNAP-tag fast in the lysosome of mammalian cell linesDepositorInsertLAMP1_STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-lamp1-mKate2
Plasmid#109507PurposeMuscle specific zebrafish expression of mKate2 fused to lamp1DepositorInsertlamp1 (lamp1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-bio-His
Plasmid#51809PurposeExpresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLAMP1 (LAMP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PGK-HA-PGRN-LAMP1TM
Plasmid#234873PurposeLentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tag and LAMP-1 transmembrane domain and cytoso…ExpressionMammalianMutationPromoterPGKAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGK-GFP-LAMP1TM
Plasmid#234874PurposeLentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM.DepositorInsertEGFP
UseLentiviralTagsLAMP-1 transmembrane domain and cytosolic tailExpressionMammalianMutationPromoterPGKAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lamp1 FLAG
Plasmid#199750PurposeLentiviral construct to stably express Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2-hSyn-G-Flamp1-mut
Plasmid#188570Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227898PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertLAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-3xHA
Plasmid#102932PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsRFP-HAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-LAMP1
Plasmid#227322PurposeDonor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-LAMP1
Plasmid#222318PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-LAMP1
Plasmid#207788PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLEX-PGK-LAMP1-HaloTag
Plasmid#164216PurposepLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsHaloTagExpressionMutationPromoterPGKAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn LAMP1-msGFP (JV012)
Plasmid#179728PurposeLentiviral expression of LAMP1-msGFP for use as a lysosomal markerDepositorInsertLAMP1 (Lamp1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-LAMP1-mCherry-iLID
Plasmid#174625PurposeOptogenetic coupling to lysosomes (LAMP1) via iLIDDepositorInsertLAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
UseTagsmCherry-iLIDExpressionMammalianMutationmCherry: Met1DelPromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Human, Homo Sapiens, Synthetic)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
rLAMP1-mCherry-EGFP-Tet-On
Plasmid#109015PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged mLAMP1 (lysosome marker)DepositorInsertrLAMP1
UseLentiviralTagsmcherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX-PGK-LAMP1-EYFP
Plasmid#164215PurposepLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsEYFPExpressionMutationPromoterPGKAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Blast-rLAMP1-eGFP
Plasmid#109028Purposestable lentiviral expression of cDNADepositorInsertrLAMP1
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZU-LAMP1-mRuby3
Plasmid#222586PurposeStable expression of LAMP1-mRuby3 in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-LAMP1-EGFP-APEX2-V5
Plasmid#236402PurposeExpresses a fluorescent lysosome-localized APEX2DepositorInsertLAMP1-EGFP-APEX2-V5
UseRetroviralTagsAPEX2, EGFP, and V5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-V5-APEX2
Plasmid#228524PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUGW-RUSH-LAMP1-mScarleti
Plasmid#228526PurposeLentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsStreptavidin Binding Protein (SBP), Streptavidin-…ExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 LAMP1-mNeonGreen HRD
Plasmid#207549PurposeHomologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cellsDepositorInsertTET inducible LAMP1-mNeonGreen
UseTagsmNeonGreenExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti sfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164478PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
mpeg:lamp1b-mCherry-stop; cmlc2:GFP
Plasmid#213739PurposeExpresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis markerDepositorInsertlamp1b-mCherry
UseZebrafishTagsExpressionMutationPromotermpeg1.1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP5380 LAMP1-SBP- mCherry-FIREtag
Plasmid#216725PurposeExpresses LAMP1-mCherry-FIREtag in mamallian cellsDepositorInsertLAMP-1 (LAMP1 Human)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-HA-PGRN-LAMP1TM-IRES-GFP
Plasmid#234877PurposeLentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tag and LAMP-1 transmembrane domain and cytoso…ExpressionMammalianMutationPromoterhSYnAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL-SBP-EGFP-LAMP1
Plasmid#222317PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAMP1-Y414A
Plasmid#222319PurposeSynchronize the trafficking of LAMP1-Y414A from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)
UseTagsExpressionMammalianMutationTyr414 to AlaPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-LAMP1-Y414A
Plasmid#222320PurposeSynchronize the trafficking of LAMP1-A17 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIG2-IRES-Lamp1-mNeon Green
Plasmid#219441PurposeExpresses Lamp1 fused to mNeon GreenDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCAGAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-035 Lamp1(D-G)//Esp3I
Plasmid#130237PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1 (Lamp1 Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-025 Lamp1-mVenus//BsaI
Plasmid#130206PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-024 Lamp1-Neptune//BsaI
Plasmid#130205PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-Neptune
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-032 Lamp1-Eos//BsaI
Plasmid#130213PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-022 Lamp1-Eos//BsaI
Plasmid#130203PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-021 Lamp1-Sirius//BsaI
Plasmid#130202PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…PromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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