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Showing: 951 - 1000 of 13473 results

  1. pCRISPR/eCas9-GPIBY55

    Plasmid
    #213709
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213709-plasmid-map-sequence-id-422949

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  4. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid
    #136140
    Purpose
    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    136140-plasmid-map-sequence-id-275306

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  23. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  25. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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  26. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  28. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

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  32. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  34. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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  39. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222498
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222498-plasmid-map-sequence-id-441135

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  40. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222499
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222499-plasmid-map-sequence-id-441136

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  41. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  42. TRE3GV-SpdCas9-Dnmt3A/3L-V1

    Plasmid
    #222503
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222503-plasmid-map-sequence-id-441222

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  43. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222505
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222505-plasmid-map-sequence-id-441223

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  44. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222506
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222506-plasmid-map-sequence-id-441224

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  49. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  50. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

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Showing: 951 - 1000 of 13473 results