We narrowed to 13,948 results for: CRISPR-Cas9

Showing: 8001 - 8040 of 13948 results

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8380

Plasmid

#126914

Purpose

Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS360

Plasmid

#73687

Purpose

Punc-47::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-47::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Promoter

Punc-47

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGNW2

Plasmid

#122086

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-5sgRNA

Plasmid

#64243

Purpose

destination vector for five U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-Simple-gRNA backbone

Plasmid

#51306

Purpose

gRNA expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Type

Empty backbone

Use

CRISPR; Xenopus expression

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cry2FL-VP64

Plasmid

#60554

Purpose

Expresses Cry2FL-VP64 in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

Cry2FL-VP64

Tags

FLAG, HA tag, and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBHM2673

Plasmid

#241720

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSD marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-4sgRNA

Plasmid

#64242

Purpose

destination vector for four U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET42b-AncBE4max

Plasmid

#165157

Purpose

Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

AncBE4max

Tags

6xHis

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA6213S

Plasmid

#122094

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Promoter

Pem7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-2sgRNA

Plasmid

#64240

Purpose

destination vector for two U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGNW6

Plasmid

#122093

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-3sgRNA

Plasmid

#64241

Purpose

destination vector for three U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGDestTol2LC-1sgRNA

Plasmid

#64239

Purpose

destination vector for one U6x:sgRNA cassette

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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vc241

Plasmid

#193453

Purpose

Expression of rRFP in A. castellanii

Depositor

Chantal Abergel

Article

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

mRFP

Use

Acanthamoeba exression

Promoter

GAPDH

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-FlpO

Plasmid

#183448

Purpose

Lentiviral FlpO vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-FlpO

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBHM2675

Plasmid

#241722

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGNW4

Plasmid

#122088

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKER-mCardinal

Plasmid

#85976

Purpose

Expresses mCardinal from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mCardinal

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-Cre

Plasmid

#183425

Purpose

Lentiviral Cre vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-Cre

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8644

Plasmid

#126919

Purpose

Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_SYCP3_nterm

Plasmid

#222524

Purpose

Cas9/sgRNA plasmid for targeting SYCP3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8787

Plasmid

#126921

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8788

Plasmid

#126922

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 8001 - 8040 of 13948 results

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We narrowed to 13,948 results for: CRISPR-Cas9