Addgene

We narrowed to 32,215 results for: IND

Showing: 10001 - 10050 of 32215 results

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pET24a-His7-NUbo-MCS

Plasmid

#212724

Purpose

Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-MCS-His6

Plasmid

#212723

Purpose

Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-NUbo-E3(63)-His6

Plasmid

#212717

Purpose

Bacterial expression of NUbo-E3(63)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Tags

6xHis and NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-NUbo-E3(48)-His6

Plasmid

#212714

Purpose

Bacterial expression of NUbo-E3(48)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Tags

6xHis and NUbo

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 hygro sgUCK1

Plasmid

#211524

Purpose

Deletes UCK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Use

Tags

TinyERRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.1-GFP

Plasmid

#209095

Purpose

GFP expressing shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-scrCtnnd2.1-mCherry-CAAX

Plasmid

#209108

Purpose

Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-shCtnnd2.1-mCherry-CAAX

Plasmid

#209107

Purpose

Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shCdh20.1 GFP

Plasmid

#209105

Purpose

GFP expressing shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh20.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCdh10.1 GFP

Plasmid

#209101

Purpose

GFP expressing shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCdh10.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.1-GFP

Plasmid

#209096

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.1

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG164

Plasmid

#202583

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TOMM20-PuRRRE

Plasmid

#208329

Purpose

PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TOMM20-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Use

Tags

PunyEEER

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSyt1C2AB(143-421)msGFP

Plasmid

#209010

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 143-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(143-421)msGFP

Plasmid

#209015

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 143-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG163

Plasmid

#202582

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/HF-CDC50B

Plasmid

#209224

Purpose

Mammalian expression of CDC50B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

CDC50B (TMEM30B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GFP-mSOX17

Plasmid

#206379

Purpose

Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-GFP-mSox17FNV

Plasmid

#206372

Purpose

Expresses EGFP fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17FNVC2

Plasmid

#206370

Purpose

Expresses mouse SOX17FNVC2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNVC2 (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17FNV

Plasmid

#206368

Purpose

Expresses mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17

Plasmid

#206367

Purpose

Expresses mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3a-UBE2G2

Plasmid

#204209

Purpose

Bacterial expression of UBE2G2

Depositor

Allan Weissman

Article

Das et al Mol Cell. 2009 Jun 26. 34(6):674-85.

Insert

UBE2G2 (Ube2g2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GreenT-EC - pRSET

Plasmid

#193947

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GreenT-EC.b - pRSET

Plasmid

#193948

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GreenT-EC.c - pRSET

Plasmid

#193949

Purpose

Bacterial expression vector for a low-affinity calcium indicator

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GFP-mSOX17FNV

Plasmid

#206380

Purpose

Expresses EGFP fused mouse SOX17FNV in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG165

Plasmid

#202584

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-TalinRod-mEmerald

Plasmid

#202382

Purpose

FKBP12-Talin(434-2541)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(434-2541)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-TalinC11-mEmerald

Plasmid

#202381

Purpose

FKBP12-Talin(1974-2541)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN12-FB(wt)

Plasmid

#202385

Purpose

mApple-Talin(1-2299)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-2299)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN3-FRB(wt)

Plasmid

#202384

Purpose

mApple-Talin(1-911)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-911)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN10-FRB

Plasmid

#202380

Purpose

mApple-Talin(1-1973)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-1973)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2084

Plasmid

#137726

Purpose

Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

Depositor

Jeremy Mottram

Article

Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

Type

Empty backbone

Use

RNAi; Trypanosoma brucei tet inducible stem loop

Tags

Expression

Mutation

Promoter

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN BFP

Plasmid

#188644

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Use

Tags

Gal4 DNA binding domain

Expression

Yeast

Mutation

Promoter

ADH

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-Cys-HA-Ubiquitin

Plasmid

#185356

Purpose

In vitro ubiquitination assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ubiquitin

Use

Tags

GST, HA(x2)

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tornado-RNA sensor for survivin

Plasmid

#185405

Purpose

circular RNA-scaffolded sensing modules for survivin

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

U6

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_H6_Halo_L172Q_KDEL_pBABEpu

Plasmid

#183690

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(L172Q)

Use

Retroviral

Tags

Expression

Mutation

L72Q in Halotag

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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