159,238 results

1. paavCAG-post-mGRASP-2A-dTomato

   Plasmid

   #34912

   Purpose

   post

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   postsynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-ER-3

   Plasmid

   #55041

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   ER (CALR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFUSEss-CHIg-mG1_M18

   Plasmid

   #82357

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. human EB1 fused to monomeric RFP

   Plasmid

   #39323

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Inserts

   EB1 (MAPRE1 Human)
   mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-EGFP-IRES-puro

   Plasmid

   #128652

   Purpose

   mammalian expression of EGFP with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Use

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-mWatermelon

   Plasmid

   #190625

   Purpose

   Bacterial expression of mWatermelon GFP

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   mWatermelon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLHDR03_mCherry_tCD19_unique guide

   Plasmid

   #124062

   Purpose

   Construction of 1st donor for scarless genome editing with unique guide

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   mCherry
   tCD19
   

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Human UbC modified

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. gGBEv6.3-SpCas9

   Plasmid

   #202629

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpCas9(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_Xph20-eGFP-CCR5TC

    Plasmid

    #135530

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-tetO-lox-hSOX2

    Plasmid

    #20729

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CCR5-DuET

    Plasmid

    #213201

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE6e

    Plasmid

    #207855

    Purpose

    Mammalian expression of PE6e prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6e
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, K918A, K775R)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV.rTH.PI.Cre.SV40

    Plasmid

    #107788

    Purpose

    AAV vector expressing Cre from rTH promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde and AAV9

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    rTH

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid

    #50460

    Purpose

    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid

    #113865

    Purpose

    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-HA-SAMTOR

    Plasmid

    #100513

    Purpose

    Expresses HA tagged SAMTOR in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmScarlet-i_C1

    Plasmid

    #85044

    Purpose

    Mammalian expression of mScarlet-i

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    mScarlet-i
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-ShadowG

    Plasmid

    #104620

    Purpose

    Expresses Shadow G in mammalian cell

    Depositor

    Hideji Murakoshi

    Article

    Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

    Insert

    ShadowG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pEGFP-p97

    Plasmid

    #85670

    Purpose

    Expression of p97 with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGWB506

    Plasmid

    #74848

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR221_SLC2A1

    Plasmid

    #132103

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A1 (SLC2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. GfaABC1D BARK D110A p2A mCherry

    Plasmid

    #117692

    Purpose

    GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs D110A peptide
    

    Use

    AAV

    Tags

    HA / FLAG

    Expression

    Mutation

    D110A

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEX-A-U6-gRNA

    Plasmid

    #65626

    Purpose

    Empty vector for the expression U6 driven gRNA

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    U6-gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HBV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTXB1.Tat(48-57)-Cre recombinase

    Plasmid

    #165042

    Purpose

    Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Mxe Intein-Chitin binding domain and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDB5320

    Plasmid

    #204830

    Purpose

    An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW2 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG364-greenFAST

    Plasmid

    #160353

    Purpose

    expresses greenFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    greenFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FLAG-SENP6m

    Plasmid

    #18716

    Depositor

    Edward Yeh

    Article

    Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

    Insert

    FLAG-SENP6m (SENP6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C1030A

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pRSFDuet-1_VNp6-mNeongreen

    Plasmid

    #182388

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFUSE2ss-CLIg-mK_M18

    Plasmid

    #82358

    Purpose

    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCasso

    Plasmid

    #207530

    Purpose

    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK-flag-GFP11

    Plasmid

    #78590

    Purpose

    Expression flag-GFP 11 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 11
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CROPseq-iT7

    Plasmid

    #211699

    Purpose

    NIS-Seq-compatible lentiviral vector for expressing sgRNAs

    Depositor

    Jonathan Schmid-Burgk

    Article

    Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG365-redFAST

    Plasmid

    #160354

    Purpose

    Expresses redFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    redFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge