165,075 results

1. HER2 CA (V659E)

   Plasmid

   #16259

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Expression

   Mammalian

   Mutation

   Constitutively active: V659E

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNR220

   Plasmid

   #79541

   Purpose

   Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   A118
   

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbA6c-RFP

   Plasmid

   #35290

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 TEV LIC cloning vector (2B-T)

   Plasmid

   #29666

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. RFP Dynamin2 K44A

   Plasmid

   #128153

   Purpose

   Expression of Dynamin 2 K44A

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

   Insert

   Dynamin 2 (Dnm2 Rat)
   

   Expression

   Mammalian

   Mutation

   K44A

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid

    #186309

    Purpose

    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg30-PDGFR
    mCherry
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-CBE6b

    Plasmid

    #215820

    Purpose

    Express CBE6b (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI (cas9 )
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opie2-PuroR

    Plasmid

    #131613

    Purpose

    Expresses PuroR and dsRed.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-HRD1

    Plasmid

    #231688

    Purpose

    Expresses human HRD1 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    HRD1 (SYVN1 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. zWnt5b

    Plasmid

    #21282

    Depositor

    Randall Moon

    Article

    Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

    Insert

    Wnt5b (wnt5b Zebrafish)
    

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmGFP-ADAR2

    Plasmid

    #117929

    Purpose

    ADAR2 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR2 (ADARB1 Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. mCherry-Fis1

    Plasmid

    #182580

    Purpose

    Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Fis1
    

    Tags

    Fis1

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. GFP-KASH2ext

    Plasmid

    #187018

    Purpose

    Control for inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2ext
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV4-Tax WT

    Plasmid

    #23284

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    Tax
    

    Expression

    Mammalian

    Available Since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pWZL Neo Myr Flag YES1

    Plasmid

    #20665

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    YES1 (YES1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pCRISPR-PE-bacteria

    Plasmid

    #172715

    Purpose

    Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    Cas9n-linker-M-MLV2
    

    Expression

    Bacterial

    Mutation

    H840A of Cas9

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti.PGK.blast-Renilla_Luciferase

    Plasmid

    #74444

    Purpose

    Control lentiviral vector expressing Renilla luciferase

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Renilla Luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. RAD21 C-tag CRISPR

    Plasmid

    #140540

    Purpose

    RAD21 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

29. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

30. pFUW-tetO-Batf3

    Plasmid

    #139837

    Purpose

    doxycycline-inducible expression of mouse Batf3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Batf3 (Batf3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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