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P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

Plasmid

#202761

Purpose

Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mC-G-LC3B-P

Plasmid

#124974

Purpose

evaluate autophagy

Depositor

Kazuhiro Oka

Article

Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

Insert

mCherry-GFP-LC3B

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha4 EGFP-N3

Plasmid

#13602

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 4 (ITGA9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-FOXM1-shRNA-84

Plasmid

#89496

Purpose

Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-U6-crRNA-empty

Plasmid

#134921

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

crRNA for CRISPR-Cas3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-NIX

Plasmid

#223731

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-BNIP3

Plasmid

#223732

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 (BNIP3 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12747 - pAAV-AiE0784m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2747)

Plasmid

#191710

Purpose

AI plasmid ALIAS: AiP12747. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCerulean-Keratin-17

Plasmid

#55379

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Keratin (KRT18 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

MESV

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-scribP305L

Plasmid

#24601

Depositor

Senthil Muthuswamy

Article

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Insert

Scribble (SCRIB Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

P305L

Promoter

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
pET/ELP-I-CAT

Plasmid

#71461

Purpose

ELP-intein purification system

Depositor

David Wood

Article

Banki et al Nat Methods. 2005 Sep;2(9):659-61.

Type

Empty backbone

Use

Tags

ELP ((VPGXG)110) and intein

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

modified bovine U6-2

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Use

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V600E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB wt

Plasmid

#19313

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

UbcP

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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