162,552 results

1. pJK-caCas9-NatMX-Neut5L-Ade2 drive

   Plasmid

   #89577

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Ade2 gene drive
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

   Plasmid

   #221349

   Purpose

   Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Biotin [IPI-M33] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

   Plasmid

   #221350

   Purpose

   Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84241

   Purpose

   Expresses ABA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-ms-Sox3

   Plasmid

   #50779

   Purpose

   retroviral expression of mouse Sox3

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox3 (Sox3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH_CMV_TFAM_mKate2

   Plasmid

   #167331

   Purpose

   Expresses mKate2 fused to full length TFAM in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Green Lindoblum

   Plasmid

   #163113

   Purpose

   Expresses biosensor for lactate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Lindolum
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPLV_C1

   Plasmid

   #186415

   Purpose

   Endogenous murine RNase inhibitor production for cell-free lysate

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

   Insert

   Murine RNase Inhibitor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG163

    Plasmid

    #124365

    Purpose

    IPTG inducible system (gfpmut2 with Ppsank) for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank

    Available since

    July 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2273

    Plasmid

    #111899

    Purpose

    ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple343 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple343 (TUBB3 MiniPromoter)

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK232 (CMV-OsTIR1-PURO)

    Plasmid

    #72834

    Purpose

    Expresses OsTIR1 under the control of the CMV promoter

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    AAVS1-CMV-OsTIR1-PURO-AAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330A_dCas9-1x4

    Plasmid

    #63598

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD_dGFPmut3-AAV

    Plasmid

    #187379

    Purpose

    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

    Depositor

    Roi Avraham

    Insert

    dGFPmut3_AAV
    

    Use

    Tags

    AAV

    Expression

    Bacterial

    Mutation

    C-terminal AAV tail

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH-AFID-3

    Plasmid

    #167361

    Purpose

    For inducing predictable deletions in rice plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hsyn-NE2h

    Plasmid

    #208687

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

    Depositor

    Yulong Li

    Has Service

    AAV5

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid

    #216152

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST2T18ms

    Plasmid

    #72647

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pC2HB

    Plasmid

    #63734

    Purpose

    bait plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans bait plasmid

    Tags

    HA, LexA, and NLS

    Expression

    Mutation

    Promoter

    Met3

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-NLS-AcrIIA4

    Plasmid

    #113037

    Purpose

    Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. SpRY-ABE8e(V106W)

    Plasmid

    #198553

    Purpose

    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TH

    Plasmid

    #39079

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TH (TH Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. AiP1427 - pAAV-AiE2135m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214497

    Purpose

    AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC2HP

    Plasmid

    #63735

    Purpose

    prey plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans prey plasmid

    Tags

    FLAG, NLS, and VP domain

    Expression

    Mutation

    Promoter

    Met3

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJRA110

    Plasmid

    #209343

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal StayGold fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge