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Showing: 6181 - 6210 of 167150 results

  4. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Expression
    Mammalian
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  5. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73037
    Purpose
    Express Nluc and copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73037-plasmid-map-sequence-id-136876

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  6. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  11. JWW-1 human chimeric antibody

    Plasmid
    #66748
    Purpose
    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.
    Depositor
    Richard Roden
    Article
    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:
    Inserts
    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    Expression
    Mammalian
    Mutation
    First 3 amino acids of rat light chain constant r…
    Promoter
    mEF1 and rEF1
    Available Since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66748-plasmid-map-sequence-id-118582

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  19. pTrc-trGPPS(CO)-LS

    Plasmid
    #50603
    Purpose
    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase
    Depositor
    Jay Keasling
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Inserts
    Limonene Synthase
    ApR
    LacIq
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    truncated codon optimized geranylpyrophosphate sy…
    Promoter
    Trc
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    50603-plasmid-map-sequence-id-77114

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  21. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  22. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Article
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available Since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    103056-plasmid-map-sequence-id-199055

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  26. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99312-plasmid-map-sequence-id-190933

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Showing: 6181 - 6210 of 167150 results