163,445 results

1. CYP51A1 (3I3K)

   Plasmid

   #25161

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CYP51A1_cw_09 (CYP51A1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acid residues 54-502

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. p3036 GST-HPV-16 E6

   Plasmid

   #10849

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   HPV16 E6 (E6 Human papillomavirus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

3. MBP-CRY1-PHR

   Plasmid

   #26035

   Depositor

   Johann Deisenhofer

   Article

   Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   CRY1 (CRY1 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pBabe puro-eIF4E

   Plasmid

   #33252

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   eIF4E (EIF4E Human)
   

   Use

   Retroviral

   Tags

   untagged

   Expression

   Mammalian

   Mutation

   unmodified

   Promoter

   SV40

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

5. KE-009

   Plasmid

   #133441

   Purpose

   Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4H (EIF4H Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRST7-ER beta (short)

   Plasmid

   #11357

   Depositor

   Donald McDonnell

   Article

   Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

   Insert

   ER beta short (ESR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

7. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pRK5M-TIMP3

   Plasmid

   #31715

   Depositor

   Rik Derynck

   Article

   Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

   Insert

   TIMP3 (TIMP3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. PP7 guide

   Plasmid

   #86168

   Purpose

   Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   PP7 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pErbB2-ECFP

    Plasmid

    #66945

    Purpose

    Mammalian expression of rat ErbB2 tagged with ECFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

    Plasmid

    #70741

    Purpose

    1 color strong reporter used in study

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    sfGFP-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMV::TVA/tRFP

    Plasmid

    #164486

    Purpose

    Dual CMV promoter plasmid to express TVA and TurboRFP

    Depositor

    Hollis Cline

    Article

    Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

    Inserts

    TVA (CD320 Chicken)
    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sCMV and sCMV IE94

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Use

    Tags

    Hexa his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3n1 (572)

    Plasmid

    #70727

    Purpose

    BetI/PhlF/SrpR BCD7 3-node v41-1

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Inserts

    srpR-ssrA
    betI-ssrA
    phlF-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4-ZfCry4

    Plasmid

    #136242

    Purpose

    Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    ZfCry4
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D196H- Please see depositor comments below

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-N 18E5

    Plasmid

    #37882

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 18 E5 (E5 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. FLT1-KDR

    Plasmid

    #86034

    Purpose

    chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

    Insert

    human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)
    

    Use

    Tags

    HA and t-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RabV CVS-N2c(deltaG)-APEX2-Venus

    Plasmid

    #73467

    Purpose

    Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    APEX2-Venus
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge