We narrowed to 2,111 results for: D10

1. Yeast_Target-AID3S

   Plasmid

   #188646

   Purpose

   Expresses Target-AID3S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Mammalian_SaAID

   Plasmid

   #188653

   Purpose

   Expresses SaAID in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nSaCas9-AID-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   pCMV

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSL690

   Plasmid

   #47753

   Purpose

   Expresses dCas9-VP64 fusion

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF-BE4Gam

   Plasmid

   #138269

   Purpose

   Expression of BE4am-Cas9 Base-editor from an EF1a promoter.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   BE4Gam
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Ef1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-myc-MmUbc7/2776

   Plasmid

   #11488

   Depositor

   Allan Weissman

   Article

   Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

   Insert

   MmUbc7 (Ube2g2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Mammalian_SaAID3S

   Plasmid

   #188651

   Purpose

   Expresses SaAID3S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   nSaCas9-AID3S--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Mammalian_SaAID2S

   Plasmid

   #188652

   Purpose

   Expresses SaAID2S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   AID2S-nSaCas9--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF:iCas9

   Plasmid

   #138267

   Purpose

   Expression of mTN3-GGS6-dCas9 (iCas9) in human.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mTN3-GGS6-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cas9-D10A, H840A

   Promoter

   Ef1a

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RhoG

    Plasmid

    #55694

    Purpose

    expression of mouse RhoG

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoG (Rhog Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pE2F3.3.1-gDNA

    Plasmid

    #113776

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

    Depositor

    Kevin White

    Insert

    E2F3 (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQTEV-STX10

    Plasmid

    #31572

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    syntaxin 10 (STX10 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-myc-MmUbc7mut/2777

    Plasmid

    #11489

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    cys 889 mutated to ser

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-KG-MmUbc7/2783

    Plasmid

    #11490

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. peSpdCas9-J23109

    Plasmid

    #113153

    Purpose

    Plasmid constitutively expressing eSpdCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpdCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A & K810A & H840A & K1003A & R…

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFOSGE_COF2G08

    Plasmid

    #70915

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Parg (Parg Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMO819

    Plasmid

    #232072

    Purpose

    Expresses Cytosine Base Editor for Mammalian Cells

    Depositor

    Franz Meitinger

    Article

    Ng et al Methods Mol Biol. 2025;2872:167-187. doi

    Insert

    Cytosine Base Editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha core promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG415Gal_Gcn4 E3GFP

    Plasmid

    #233008

    Purpose

    Galactose iduced expression of Gcn4 E+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 E+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D105E, D118E, D139E

    Promoter

    Gal1

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG415Gal_Gcn4 Aro3+

    Plasmid

    #232983

    Purpose

    Galactose iduced expression of Gcn4 Aro3+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAG415Gal_Gcn4 Aro3+HA

    Plasmid

    #232990

    Purpose

    Galactose iduced expression of Gcn4 Aro3+HA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG415Gal_Gcn4 Aro3+GFP

    Plasmid

    #233002

    Purpose

    Galactose iduced expression of Gcn4 Aro3+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAG415Gal_Gcn4 R3W

    Plasmid

    #232975

    Purpose

    Galactose iduced expression of Gcn4 R++ W+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 R++ W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103R, N126W, D133R, K143R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG415Gal_Gcn4 E3HA

    Plasmid

    #232996

    Purpose

    Galactose iduced expression of Gcn4 E+HA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 E+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D105E, D118E, D139E

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDest17_Gcn4 solvvol W

    Plasmid

    #231878

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol W
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDest17_Gcn4 solvvol

    Plasmid

    #231877

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQ265_BasA3G

    Plasmid

    #225145

    Purpose

    Gateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Inserts

    BasA3G-zCas9(D10A)-UGI
    -
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQ265_EtA3C

    Plasmid

    #225146

    Purpose

    Gateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    EtA3C-zCas9(D10A)-UGI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQ265_DlA3G

    Plasmid

    #225148

    Purpose

    Gateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    DlA3G-zCas9(D10A)-UGI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ265_LoA3GX1

    Plasmid

    #225149

    Purpose

    Gateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

    Insert

    LoA3GX1-zCas9(D10A)-UGI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMO1170

    Plasmid

    #232073

    Purpose

    Expresses Adenine Base Editor for Mammalian Cells

    Depositor

    Franz Meitinger

    Article

    Ng et al Methods Mol Biol. 2025;2872:167-187. doi

    Insert

    Adenine Base Editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha core promoter

    Available since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Yeast_Target-AID2S

    Plasmid

    #188647

    Purpose

    Expressing Target-AID2S in yeasts

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Yeast

    Mutation

    D10A for SpCas9

    Promoter

    pGal1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEAF1.2.2-gDNA

    Plasmid

    #113799

    Purpose

    CRISPR plasmid for expression of Cas9 nicakse (D10A) and gRNA targeting human transcription factor DEAF1

    Depositor

    Kevin White

    Insert

    DEAF1 (DEAF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDEAF1.2.1-gDNA

    Plasmid

    #112449

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1

    Depositor

    Kevin White

    Insert

    DEAF1 (DEAF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TAL3591

    Plasmid

    #47310

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-yjefn3-right (si:ch211-212d10.3 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

36. TAL3590

    Plasmid

    #47309

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-yjefn3-left (si:ch211-212d10.3 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pJK384.4

    Plasmid

    #155369

    Purpose

    dCas9 + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pH-ePPE

    Plasmid

    #183097

    Purpose

    For plant prime editing in rice plants

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AICSDP-19:LAMP1-mEGFP

    Plasmid

    #101782

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

    Depositor

    Allen Institute for Cell Science

    Insert

    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pcDNA3-Tet1

    Plasmid

    #60938

    Purpose

    Expresses Tet1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Use

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. ePPE

    Plasmid

    #183095

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

    Insert

    Nls-nCas9-NC-nls-MLV-Nls
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHAGE EF1α dCas9-KRAB

    Plasmid

    #50919

    Purpose

    Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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