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177,170 results

Showing: 10501 - 10550 of 177170 results

  1. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83475-plasmid-map-sequence-id-358352

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  3. pLV_Zika_NS2B_Flag

    Plasmid
    #79637
    Purpose
    Third generation Self-inactivating lentivector expressing NS2B from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS2B
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    79637-plasmid-map-sequence-id-151178

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  9. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid
    #75141
    Purpose
    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    fragment encoding amino acids 711-1034
    Promoter
    CMV
    Available since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    75141-plasmid-map-sequence-id-148062

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  12. pCMV6 SMCR8 HA

    Plasmid
    #74158
    Purpose
    Express SMCR8 HA tagged in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    SMCR8 (SMCR8 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74158-plasmid-map-sequence-id-139169

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  13. pKHR7

    Plasmid
    #74624
    Purpose
    a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.
    Depositor
    David Grunwald
    Publication
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cmlc2::mCherry
    Use
    Zebrafish expression
    Promoter
    cmlc2
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74624-plasmid-map-sequence-id-204495

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  15. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    74215-plasmid-map-sequence-id-139305

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  21. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    68416-plasmid-map-sequence-id-120194

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  22. GCGR-Tango

    Plasmid
    #66291
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCGR (GCGR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  25. Beta-2-adrenergic receptor-YFP

    Plasmid
    #67146
    Purpose
    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    67146-plasmid-map-sequence-id-121580

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  26. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66080-plasmid-map-sequence-id-436984

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  28. pcDNA3-TORCAR-NLS

    Plasmid
    #64931
    Purpose
    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NLS (EIF4EBP1 Human, Synthetic)
    Tags
    Cerulean, Nuclear localization signal (NLS), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64931-plasmid-map-sequence-id-109697

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  30. GFP-ATAD2

    Plasmid
    #65370
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATAD2 (ATAD2 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65370-plasmid-map-sequence-id-177053

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  33. pSBtet-GB

    Plasmid
    #60504
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60504-plasmid-map-sequence-id-97543

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  36. pAMPK alpha2 delta312X

    Plasmid
    #60127
    Purpose
    expresses constitutively active AMPKalpha2
    Depositor
    Morris Birnbaum
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPK alpha 2 (Prkaa2 Rat)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Truncated at residue 312
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    60127-plasmid-map-sequence-id-317039

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  40. mCherry-PCNA-19-SV40NLS-4

    Plasmid
    #55117
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. mOrange-pBAD

    Plasmid
    #54751
    Purpose
    Localization: Protein Expression Vector , Excitation: 548, Emission: 562
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and mOrange
    Expression
    Bacterial
    Available since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    54751-plasmid-map-sequence-id-453673

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  47. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  48. mRuby-N1

    Plasmid
    #54581
    Purpose
    Localization: N1 Cloning Vector, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRuby
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    54581-plasmid-map-sequence-id-393318

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Showing: 10501 - 10550 of 177170 results