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173,273 results

Showing: 10601 - 10650 of 173273 results

  1. pQCXIP-cathepsinD-YMH

    Plasmid
    #182644
    Purpose
    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    cathepsinD
    Use
    Retroviral
    Tags
    His6-tag, TS site from proCCK, and myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182644-plasmid-map-sequence-id-358628

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  8. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Available Since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    87067-plasmid-map-sequence-id-192710

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  12. nLuc_AP4_TEVs_P3mS

    Plasmid
    #119299
    Purpose
    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    Use
    Luciferase
    Tags
    Myc tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119299-plasmid-map-sequence-id-231827

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  16. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

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  17. pGL4 [luc2P/Gli-RE/Hygro] Vector

    Plasmid
    #236803
    Purpose
    Express Gli response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    Gli (GLI1 Human)
    Use
    Luciferase
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236803-plasmid-map-sequence-id-471813

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  18. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Expression
    Bacterial
    Mutation
    A309G
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

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  20. YopH-pNIC-Bsa4

    Plasmid
    #92063
    Purpose
    pNIC expression vector encoding gene for YopH
    Depositor
    Josh Beckham
    Insert
    YopH
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Codon optimized E. coli
    Available Since
    Jan. 17, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    92063-plasmid-map-sequence-id-233162

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  23. TFORF1759

    Plasmid
    #143845
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ALYREF (ALYREF Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143845-plasmid-map-sequence-id-396272

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  29. R777-E317 Hs.SOS1

    Plasmid
    #70601
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70601-plasmid-map-sequence-id-358354

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  33. TFORF0070

    Plasmid
    #141505
    Purpose
    Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CSDE1 (CSDE1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    141505-plasmid-map-sequence-id-454897

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  36. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Expression
    Bacterial
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  37. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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  40. pLAS2w.Phyg-HAVCR1

    Plasmid
    #232669
    Purpose
    expresses HAVCR1 in mammalian cells
    Depositor
    Chen-Yang Shen
    Insert
    HAVCR1 (HAVCR1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232669-plasmid-map-sequence-id-464304

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  43. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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  46. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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  48. EBFP2-Beta-Catenin-20

    Plasmid
    #55235
    Purpose
    Localization: Tight Junctions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10601 - 10650 of 173273 results