165,256 results

1. TALED_Left-Cox3.1-1397N.

   Plasmid

   #212950

   Purpose

   Expresses TALED_Left-Cox3.1-1397N. in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

   Insert

   TALED_Left-Cox3.1-1397N
   

   Expression

   Mammalian

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR223-LMP1

   Plasmid

   #195805

   Purpose

   Gateway Entry Clone of Epstein-Barr virus LMP1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   LMP1 (LMP-1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTagRFP657-N1

   Plasmid

   #31959

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Expression

   Mammalian

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LAMP1-SBP-GFP

   Plasmid

   #120172

   Purpose

   Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Lysosomal-associated membrane protein 1 (Lamp1 Rat)
   

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBS hTSP-5

   Plasmid

   #13002

   Depositor

   Jack Lawler

   Article

   Newton et al Genomics. 1994 Dec . 24(3):435-9.

   Insert

   COMP (COMP Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

   Plasmid

   #154987

   Purpose

   Lentiviral vector expressing Human ACE2 and TMPRSS2

   Depositor

   Sonja Best

   Insert

   ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO-perox-tagRFP

   Plasmid

   #185600

   Purpose

   Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   perox-tagRFP-T
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rogi2-CMV-mRuby-bGH

   Plasmid

   #179861

   Purpose

   Targets Rogi2 genomic region with HDR and expresses mRuby

   Depositor

   Sai Reddy

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   mRuby
   

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Beta-2-adrenergic receptor-CFP

    Plasmid

    #55794

    Purpose

    Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-ECFP (ADRB2 Human, Aequorea victoria)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid

    #111744

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q30 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    T1413I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid

    #220349

    Purpose

    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

    Depositor

    Tomoko Igawa

    Article

    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

    Inserts

    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    

    Tags

    Superman Repression Domain X (SRDX) and VP16 tran…

    Expression

    Plant

    Mutation

    two amino acid substitution in WUS-box: L255A L25…

    Promoter

    CaMV 35S and RPS5A

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SSTR2-Tango

    Plasmid

    #66503

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR2 (SSTR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eGFP L202 Reporter

    Plasmid

    #119129

    Purpose

    Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    mCherry and eGFP
    

    Use

    Lentiviral

    Mutation

    eGFP L202S

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-IRES-Hygro_GCaMP6s

    Plasmid

    #164591

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid

    #213899

    Purpose

    Imaging of KIF5B fused to HaloTag

    Depositor

    Lukas Kapitein

    Insert

    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCONR221

    Plasmid

    #216146

    Purpose

    Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

    Depositor

    Imran Siddiqi

    Article

    Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

    Type

    Empty backbone

    Use

    Cloning backbone

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

25. Lenti-sgNT.2#2/Cre

    Plasmid

    #173661

    Purpose

    Expresses a NT.2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF3305

    Plasmid

    #144781

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCold-TF-hRubicon

    Plasmid

    #64147

    Purpose

    For protein expression in E. coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    Rubicon (RUBCN Human)
    

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

29. WT-MEK1-V5

    Plasmid

    #217297

    Purpose

    Lentivirus vector for expression of MEK1

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TagBFP2-PIP4K2A

    Plasmid

    #202726

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2A (PIP4K2A Human)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Tags

    tandem EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP-RAPH1ΔTBS

    Plasmid

    #194859

    Purpose

    Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 2-92 deleted

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FLAG-mBAF45a IRES GFP

    Plasmid

    #17880

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45a (Phf10 Mouse)
    

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR223-ADRBK1

    Plasmid

    #23835

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF2161

    Plasmid

    #141950

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Human, Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti-CMV-FlaviA-mNeptune-puro

    Plasmid

    #140091

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-mNeptune
    

    Use

    Lentiviral; High expression

    Tags

    mNeptune

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid

    #214265

    Purpose

    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

    Depositor

    D. James Surmeier

    Insert

    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Synapsin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLenti-C-Myc-DDK-IRES-Puro HNRNPQ

    Plasmid

    #200729

    Purpose

    Lentiviral expression of Flag-Tagged human HNRNPQ

    Depositor

    Claudio Alarcón

    Insert

    HNRNPQ (SYNCRIP Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pOPINVHH_his_flag

    Plasmid

    #210404

    Purpose

    For expression of VHH with C-terminal his and Flag tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Tags

    his6-flag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSD5

    Plasmid

    #197289

    Purpose

    Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans

    Depositor

    Bogumil Karas

    Article

    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

    Insert

    NptII
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. TRUPATH Triple GaZ

    Plasmid

    #196053

    Purpose

    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pUPD2 RDF ( GB1498)

    Plasmid

    #160579

    Purpose

    Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge