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167,150 results

Showing: 10601 - 10650 of 167150 results

  6. Cd2ap-GFP

    Plasmid
    #221528
    Purpose
    Fluorescently tagged Cd2ap-GFP
    Depositor
    Alessandro Ori
    Insert
    Cd2ap (CD2AP Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221528-plasmid-map-sequence-id-439382

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  9. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  10. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  14. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Expression
    Mammalian
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1733-plasmid-map-sequence-id-334

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  23. WT Smarca4-V5 IRES-puro

    Plasmid
    #107058
    Purpose
    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107058-plasmid-map-sequence-id-201960

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  24. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    tight TRE promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

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  25. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid
    #29663
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, GFP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29663-plasmid-map-sequence-id-197189

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  37. plex_307-ccdB-g418

    Plasmid
    #201141
    Purpose
    pLEX_307 plasmid with g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201141-plasmid-map-sequence-id-395514

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  44. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  50. pdCas9-humanized

    Plasmid
    #44246
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dead Cas9 with 3X NLS
    Use
    CRISPR and Retroviral; Vector
    Tags
    3x NLS
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44246-plasmid-map-sequence-id-60247

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Showing: 10601 - 10650 of 167150 results