We narrowed to 12,055 results for: cel.2

1. pDonor-Prf1-ires-mCherry

   Plasmid

   #209073

   Purpose

   Targeting vector for the mouse Prf1 locus to correct prf1 gene

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV_TRET_Ngn2‐2A‐Ngn1

   Plasmid

   #61471

   Purpose

   3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn2 (Neurog2 Mouse)
   Neurog1 (Neurog1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF.delmFasL.CMV.GFP

   Plasmid

   #17620

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Insert

   delFasL (Fasl Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Noncleavable mouse delFasL . Deleted the exon 2 r…

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJR85

   Plasmid

   #140095

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pECE-HA-AMPKalpha2 wt

   Plasmid

   #31654

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
   

   Use

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-cT3G-cERP2-WT1

   Plasmid

   #192906

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of WT1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pECE-M2-AMPKalpha2 wt

   Plasmid

   #31652

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pOGS539_GLuc-ns

   Plasmid

   #226719

   Purpose

   Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW57.1-Cdx2-3xT7

    Plasmid

    #200891

    Purpose

    Inducible lentiviral expression of Cdx2

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Cdx2 (Cdx2 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK-ATF4 1-275

    Plasmid

    #26118

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 276-351 have been deleted

    Promoter

    Available Since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pPB-TetON-mEGFP-NGN2_WT

    Plasmid

    #215610

    Purpose

    For integration of NGN2 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRK-ATF4 111-351

    Plasmid

    #26119

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 1-110 have been deleted

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRK-ATF4 248-351

    Plasmid

    #26120

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ATF4 aa's 1-247 have been deleted

    Promoter

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pPB-cT3G-cERP2-ZFPM2

    Plasmid

    #192922

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-PE VQR

    Plasmid

    #162797

    Purpose

    All-in-one prime editor piggyBac transposon, VQR variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    CAG

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HOXB5 (human) FLAG-MSCV

    Plasmid

    #8532

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGEX PLCg2(NC)-SH2

    Plasmid

    #46472

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg2(NC) (PLCG2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 524-749

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECE-M2-BAIAP2 S366A

    Plasmid

    #31657

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 S366A (BAIAP2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 366 to Alanine

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pIRES2-eGFP:HA-mTREK-1(S131C/A286F)

    Plasmid

    #224775

    Purpose

    Mammalian expression vector for generating mouse TREK-1

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S131C / A286F

    Promoter

    CMV

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEMHE:mTREK-1 (S131A/C159A/C219A)

    Plasmid

    #224753

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A/C159A/C219A

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMHE:mTREK-1 (S131C/C159A/C219A)

    Plasmid

    #224752

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C/C159A/C219A

    Promoter

    T7

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

    Plasmid

    #224754

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    C159A/C219A/C365A/C399A

    Promoter

    T7

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330_DAZL_cterm2

    Plasmid

    #222919

    Purpose

    Cas9/sgRNA plasmid for targeting DAZL

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMAL-MBP-KRas-Q61L

    Plasmid

    #214300

    Purpose

    Express constitutively active KRas fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active KRas (KRAS Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    KRas-Q61L

    Promoter

    Tac

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMAL-MBP-KRas-S17N

    Plasmid

    #214301

    Purpose

    Express dominant negative KRas fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Dominant negative KRas (KRAS Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    KRas-S17N

    Promoter

    Tac

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330_FIGLA_cterm2

    Plasmid

    #222907

    Purpose

    Cas9/sgRNA plasmid for targeting FIGLA

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, FIGLA sgRNA 2 (FIGLA Human, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGAL4-DBD-NGN2-WT

    Plasmid

    #215607

    Purpose

    For overexpression of Gal4-DBD NGN2 WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDonor-Prf1-mock-ires-mCherry

    Plasmid

    #209074

    Purpose

    Targeting vector for the mouse Prf1 locus to correct prf1 gene

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSIREN-RetroQ-HSPE-sh2

    Plasmid

    #92034

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to HPSE

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #2 to HPSE (Hpse Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pCDNA3.1+ HA-hAO7

    Plasmid

    #137840

    Purpose

    N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 (RNF25 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CS-TRE-BMI1-Ubc-rtTA

    Plasmid

    #198191

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBMI1 (BMI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPB-cT3G-cERP2-RUNX2

    Plasmid

    #192926

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR-li-p33_RS-CLIP

    Plasmid

    #104634

    Purpose

    Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

    Depositor

    Sébastien Walchli

    Article

    Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

    Insert

    modified Invariant chain p33 (CD74 Human)
    

    Use

    Entry vector for gateway system

    Tags

    none

    Expression

    Mutation

    CLIP domain was replaced by 2 restriction sites

    Promoter

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB-PEmax SpG

    Plasmid

    #187895

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Str-KDEL_CD63-SBP-mCherry

    Plasmid

    #222326

    Purpose

    Synchronize the trafficking of CD63 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCX-DI-2A

    Plasmid

    #74671

    Purpose

    Mammalian expression of DI-2A (hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

    Inserts

    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Use

    Tags

    F2A

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. tetO-ESRRA

    Plasmid

    #170683

    Purpose

    doxycycline-inducible overexpression of ESRRA

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRA (ESRRA Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCW57.1-Eomes-3xT7

    Plasmid

    #200888

    Purpose

    Inducible lentiviral expression of Eomes

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. PH-Btk-mRuby2

    Plasmid

    #124657

    Purpose

    PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    BTK (BTK Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    contains only the PH domain of Bruton tyrosine ki…

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. PB-PEmax NG

    Plasmid

    #187894

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. MSCV p2GM AMPK alpha2hp1 alpha1hp1

    Plasmid

    #89492

    Purpose

    shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    2 hairpins against mouse/human AMPKalpha1 and alpha2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

47. PA-mCherry-miniSOG-Mito-7

    Plasmid

    #57795

    Purpose

    Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. HA-HIF2α P405A/P531A-pBabe-Hygro

    Plasmid

    #21676

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids from wt: Proline 405 t…

    Promoter

    Available Since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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49. pS6-TGFB2-H8

    Plasmid

    #128502

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (L21-S414) (TGFB2 Human)
    

    Use

    Tags

    His tag (6x)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. PB-PE xCas9

    Plasmid

    #172652

    Purpose

    All-in-one prime editor piggyBac transposon (xCas9)

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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