We narrowed to 26,454 results for: CAL

1. pOPO640

   Plasmid

   #195296

   Purpose

   pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   miniMcCAST (Tn7575) with KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK300

   Plasmid

   #87766

   Purpose

   empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

   Plasmid

   #164266

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6 promoter from W05B2.8

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSiP1

   Plasmid

   #21952

   Depositor

   Klaus Rajewsky

   Article

   Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

   Insert

   Pten (Pten Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2010

   Availability

   Academic Institutions and Nonprofits only

5. LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

   Plasmid

   #104117

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   QuasAr3-dark Citrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin I

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK2_008

   Plasmid

   #123703

   Purpose

   Encodes human pUbc as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   phUbC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEG BacMam N term myc eGFP 3C

   Plasmid

   #160682

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   myc eGFP 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HcKan_T

   Plasmid

   #65316

   Purpose

   receiving vector of terminators for the construction of standard terminator parts in YeastFab work

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTrichoGate-5

   Plasmid

   #160705

   Purpose

   Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   Trichoderma optimised Synthetic eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Flag-Tpr-Si

    Plasmid

    #60918

    Purpose

    Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

    Depositor

    Vinay Nandicoori

    Article

    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

    Insert

    TPR (TPR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations in siRNA target region; E1…

    Promoter

    CMV

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. actc1b-mKate2-rab34a

    Plasmid

    #109625

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab34a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab34a (rab34a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. actc1b-actn2-mKate2

    Plasmid

    #109751

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    alpha actinin 2
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HcKan_O

    Plasmid

    #65315

    Purpose

    receiving vector of ORFs for the construction of standard ORF parts in YeastFab work

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HcKan_P

    Plasmid

    #65314

    Purpose

    receiving vector of promoters for the construction of standard promoter parts in YeastFab work

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNB330

    Plasmid

    #60147

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAC_C_TR61B

    Plasmid

    #111679

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    TR61B (TRMT61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 274_pSKB3-Venus-LUMP

    Plasmid

    #65976

    Purpose

    Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Insert

    LUMP
    

    Use

    Tags

    Venus and thrombin site (11-residue linker that h…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JPF0512

    Plasmid

    #124003

    Purpose

    Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK3_027

    Plasmid

    #123742

    Purpose

    Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VP16::PYLcs-T2A-GAL4DBD::ABIcs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32474

    Purpose

    Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3 HC
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pL59-Nat

    Plasmid

    #140465

    Purpose

    CRISPR/Cas9 engineering with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCV-puro-5A, 6A mMCL1-OM

    Plasmid

    #45818

    Depositor

    Joseph Opferman

    Article

    Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

    Insert

    5A, 6A mMCL-1-OM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Arginine to Alanine at 5th and 6th amino …

    Promoter

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid

    #121961

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    R911A

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. actc1b-mKate2-rab5c

    Plasmid

    #109651

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab5c

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab5c (rab5c Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. actc1b-mKate2-rab11ba

    Plasmid

    #109596

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab11ba

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab11ba (rab11ba Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUAS-kkv-mos::NG

    Plasmid

    #138958

    Purpose

    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv (NEWENTRY Fly)
    

    Use

    Uas transgene vector

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    8 bp that are in the region that encodes the cata…

    Promoter

    UAS

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTrichoGate-11

    Plasmid

    #160711

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. N6AMT1_pCSdest

    Plasmid

    #53843

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    N6AMT1 (N6AMT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

30. mCerulean3-Zyxin-6

    Plasmid

    #55454

    Purpose

    Localization: Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pETM33_OXSR1_CCT

    Plasmid

    #178482

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    OXSR1_OXSR1 (OXSR1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. actc1b-mKate2-EHD2a

    Plasmid

    #109516

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to EHD2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD2a (ehd2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ARB342

    Plasmid

    #124025

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. cr3Dd

    Plasmid

    #184862

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Syt2

    Plasmid

    #186688

    Purpose

    Expresses Synaptotagmin 2 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Synaptotagmin 2
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. JPF0511

    Plasmid

    #124002

    Purpose

    Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_phubc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. JPF0520

    Plasmid

    #124011

    Purpose

    Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHH0103_SRPK2_Pkinase

    Plasmid

    #110026

    Purpose

    Protein expression and purification of SRPK2_Pkinase

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SRPK2_Pkinase (SRPK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBEAST2-sfGFP

    Plasmid

    #126575

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge