We narrowed to 17,391 results for: puro

1. GFP-RNF110

   Plasmid

   #126547

   Purpose

   Expresses GFP-tagged RNF110 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF110 (PCGF2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PLK1 G6.1 gRNA

   Plasmid

   #90835

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK1 (Guide Designation G6.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEBTet-SNAP-plk4

   Plasmid

   #136788

   Purpose

   Mammalian expression of the centrosomal protein plk4 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-plk4 (PLK4 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDECKO-mCherry MALAT1_Enhancer.1

   Plasmid

   #78536

   Purpose

   Experimental plasmid

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ATAD2 D8.3 gRNA

   Plasmid

   #90539

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ATAD2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ATAD2 (Guide Designation D8.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-PD-L1 3SA/4NQ

   Plasmid

   #121491

   Purpose

   Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IPU-FLAG-NEK9T210A

   Plasmid

   #168270

   Purpose

   Expresses kinase-dead NEK9 mutant in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 210 to alanine

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLJM1 FRB-myc-RagA

   Plasmid

   #112740

   Purpose

   express FRB-myc-RagA

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   FRB-myc-RagA (RRAGA Human)
   

   Use

   Lentiviral

   Tags

   FRB, myc

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FOXM1 C11.2 gRNA

   Plasmid

   #90690

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FOXM1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FOXM1 (Guide Designation C11.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SMC3 A8.4 gRNA

    Plasmid

    #90897

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC3 (Guide Designation A8.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SMC3 A7.4 gRNA

    Plasmid

    #90896

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC3 (Guide Designation A7.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SMC3 H8.1 gRNA

    Plasmid

    #90895

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC3 (Guide Designation H8.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SMC3 H7.1 gRNA

    Plasmid

    #90894

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC3 (Guide Designation H7.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Clim-DC2-NMM-tdtomato

    Plasmid

    #129557

    Purpose

    pClimDC-NMM:tdT cell membrane marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    N-Myristoylation motif (NMM) fused to tdtomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMP-EED_2

    Plasmid

    #36386

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EED (EED Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MO70-hCRACR2A-isoform-c

    Plasmid

    #79591

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

    Insert

    CRACR2A-isoform-c (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

17. WEE1 F10.4 gRNA

    Plasmid

    #90941

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    WEE1 (Guide Designation F10.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WEE1 F9.4 gRNA

    Plasmid

    #90940

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human WEE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    WEE1 (Guide Designation F9.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLNCX2-Orai1

    Plasmid

    #89818

    Purpose

    Express Orai1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. POT1 E3.4 gRNA

    Plasmid

    #90842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human POT1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    POT1 (Guide Designation E3.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXSIP-GPR35-FLAG-APEX

    Plasmid

    #204706

    Purpose

    expresses GPR35-FLAG-APEX in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Use

    Tags

    FLAG, APEX

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXPR_sgGFP4

    Plasmid

    #119876

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLIX402 caPHF2

    Plasmid

    #91701

    Purpose

    inducible lentiviral expression of constitutively active PHF2

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    PHF2 (PHF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    caPHF2; S757D, S899D, S954D, S1056D

    Promoter

    TRE promoter, Tet ON

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

24. CEP55 B12.2 gRNA

    Plasmid

    #90623

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CEP55

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CEP55 (Guide Designation B12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CEP55 B11.2 gRNA

    Plasmid

    #90622

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CEP55

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CEP55 (Guide Designation B11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-CAGGS-mNAGA

    Plasmid

    #196448

    Purpose

    Expresses modified α-N-acetylgalactosaminidase in mammalian cells

    Depositor

    Yuichiro Miyaoka

    Article

    Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

    Insert

    modified alpha-N-acetylgalactosaminidase (NAGA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL421_MLL_promoter

    Plasmid

    #118695

    Purpose

    Express MLL/KMT2A promoter for luciferase assay

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    MLL_promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    MLL/KMT2A promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSMP-SETDB1_2

    Plasmid

    #36348

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

30. p7612 MSCV-P C-FlagHA 16E7 E10K

    Plasmid

    #163303

    Purpose

    Expresses HPV16 E7 E10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 E10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    E10K

    Promoter

    MSCV LTR

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p6897 PHAGE-P CMVT N-HA-hE6AP III wt

    Plasmid

    #37605

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP III (UBE3A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV tet regulatable

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

32. CCNA2 B2.1 gRNA

    Plasmid

    #90575

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCNA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCNA2 (Guide Designation B2.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CCNA2 B1.1 gRNA

    Plasmid

    #90574

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCNA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCNA2 (Guide Designation B1.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLJM1 FRB-myc-RagC

    Plasmid

    #112741

    Purpose

    express FRB-myc-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CLTC G9.4 gRNA

    Plasmid

    #90634

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CLTC

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CLTC (Guide Designation G9.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. KIF11 F5.3 gRNA

    Plasmid

    #90716

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF11

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF11 (Guide Designation F5.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MSCV FLIP p53

    Plasmid

    #19745

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FOXM1 C12.2 gRNA

    Plasmid

    #90691

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FOXM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FOXM1 (Guide Designation C12.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSMP-DNMT3A_1

    Plasmid

    #36380

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

40. BRD4 CRISPRi plasmid

    Plasmid

    #154890

    Purpose

    Human BRD4 CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    BRD4-targeting gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. RNASEH2A B10.3 gRNA

    Plasmid

    #90879

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEH2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RNASEH2A (Guide Designation B10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. lentiCRISPR.sgKras.9

    Plasmid

    #91894

    Purpose

    sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

    Depositor

    Wen Xue

    Article

    Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Kras sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. DHCR7 B6.3 gRNA

    Plasmid

    #90651

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DHCR7

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DHCR7 (Guide Designation B6.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. DHCR7 B5.3 gRNA

    Plasmid

    #90650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DHCR7

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DHCR7 (Guide Designation B5.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDECKO-mCherry MALAT1_Exon.1

    Plasmid

    #78540

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106354

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. TOP2A G11.2 gRNA

    Plasmid

    #90914

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TOP2A (Guide Designation G11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. PLKO.1-UPF3B-CDS

    Plasmid

    #136045

    Purpose

    UPF3B shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GAAGCCTTGTTCCGATCTAAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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