We narrowed to 16,474 results for: por

1. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194687

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OK+9MS-Cas9

   Plasmid

   #120354

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIP-EGFP-CDMPR

   Plasmid

   #182642

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   CDMPR
   

   Use

   Retroviral

   Tags

   EGFP and N-terminal ER targeting sequence of hema…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLacO-pEF1α-RFP

   Plasmid

   #179507

   Purpose

   Expresses monomeric RFP in mammalian cells under a EF1α promoter

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   monomeric RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLGB36

   Plasmid

   #135621

   Purpose

   Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

   Depositor

   Laurie Comstock

   Article

   Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

   Insert

   bfe3
   

   Use

   Bacteroides suicide vector with inducible counter…

   Tags

   signal sequence for periplasmic targeting

   Expression

   Mutation

   Promoter

   Bacteroides promoter regualted by TetR transcript…

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hAAVS1-gs4_EF1a-Cas9-2A-GFP

   Plasmid

   #118414

   Purpose

   CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   gRNA AAVS1 (AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCB619

   Plasmid

   #171011

   Purpose

   CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   U6:sgRNA_Puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCU-mCherry

   Plasmid

   #232790

   Purpose

   Encodes an mCherry-tagged MCU/Mitochondrial Calcium Uniporter

   Depositor

   Giulia Bertolin

   Article

   Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

   Insert

   Calcium uniporter protein, mitochondrial (MCU )
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-Rab8a-wt

   Plasmid

   #86078

   Purpose

   A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Cryptococcus Histone3 promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-uTEV1-CB5

    Plasmid

    #171007

    Purpose

    HiLITR protease with CB5 C-terminal tmd targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-CB5(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215328

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    FOXJ1 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXJ1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PMXS-SLC1A3

    Plasmid

    #72873

    Purpose

    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    SLC1A3 (SLC1A3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLHCX-FLAG-mPKM2

    Plasmid

    #44239

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M2 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GEF-H1 FLARE212.pa

    Plasmid

    #134930

    Purpose

    GEF-H1 biosensor that does not have GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TetO-FUW-Rfx4

    Plasmid

    #61544

    Purpose

    Expresses Rfx4 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Rfx4 (Rfx4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-ROR2-V5/HIS

    Plasmid

    #205608

    Purpose

    Expression of human ROR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR2 receptor tyrosine kinase (ROR2 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcpBpaRS_v2

    Plasmid

    #196485

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. GFP-p38beta

    Plasmid

    #86833

    Purpose

    mammalian expression of GFP-p38beta

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38beta (MAPK11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174883

    Purpose

    Targeting vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Pou5f1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR221_SLC6A3

    Plasmid

    #132160

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

    Insert

    SLC6A3 (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hajime Hirase, Hirokazu Hirai

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

    Plasmid

    #177265

    Purpose

    STREAMING-tag cassette

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-myc-FLNa S2152A

    Plasmid

    #8983

    Depositor

    John Blenis

    Article

    Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

    Insert

    Filamin A S2152A (FLNA Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Ser2152Ala

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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