Skip to main content
Addgene
Showing: 441 - 460 of 26683 results
  1. pET11a asyn Q79N (Q109H) (TAC-->TAT)

    Plasmid
    #105736
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Insert
    alpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q79N/Q109H
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid
    #210767
    Purpose
    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
    Depositor
    Insert
    SREBP-1 (SREBF1 Human)
    Use
    Tags
    E-tag and T7x2
    Expression
    Mammalian
    Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent m…
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

    Plasmid
    #120404
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine
    Depositor
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Containing complete variable region with a mutati…
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid
    #105738
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Insert
    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q79N/Q109N
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid
    #101349
    Purpose
    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3
    Depositor
    Insert
    NL4-3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    delta tat delta TAR delta env GFP in Nef Tet ope…
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 441 - 460 of 26683 results