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Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Fascin-Promoter 402 mutation
Plasmid#89825PurposeFascin promoter for luciferase assayDepositorInsertFascin1 (FSCN1 Human)
UseLuciferaseTagsExpressionMutation-370 Smad binding element mutated from CAGAC to T…PromoterSV40Available sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N (Q109H) (TAC-->TAT)
Plasmid#105736PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79N/Q109HPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)
Plasmid#14846DepositorInsertPHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain EE mutation
Plasmid#58833PurposeExpresses GFP-Tagged and mutated CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationchanged Arginine 43 to GlutamatePromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3 R262W (disease-associated mutation)
Plasmid#218647PurposeGateway shuttling vector for cloning NLRP3-R262W (no stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsExpressionMutationR262WPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)
Plasmid#210767PurposeMammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationDRSR 514-517 to AS; 557 R to A; multiple silent m…PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
UseTagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated N-terminal (N) O-Glycosylation site
Plasmid#120404Purposeenables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContaining complete variable region with a mutati…PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N Q109N (TAC-->TAT)
Plasmid#105738PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79N/Q109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)
Plasmid#14849DepositorInsertGAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
UseTagsVYFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)
Plasmid#14850DepositorInsertGAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-A1T6. TATA box mutated from TATATAAG to AATA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163055PurposeGrx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT
Plasmid#132667PurposeExpresses hypoxia-inducible Cas9 and TdT in mammalian cells.DepositorInsertCas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationPromoter4xHRE_YB TATAAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mammalian expression: Z-lock cofilin dark state mutant
Plasmid#141136Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef
Plasmid#126553PurposeReplication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsThy1.2 and GFPExpressionMutationPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
E. coli expression: Z-lock cofilin dark state mutant
Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionBacterialMutationD3 deletion, I32F in Zdk2PromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta tat delta TAR delta env GFP in Nef Tet ope…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only