We narrowed to 15,858 results for: cas9

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HiFi Cas9

Plasmid

#188490

Purpose

Expresses FLAG tagged HiFi Cas9

Depositor

Isaac Hilton

Publication

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R691A

Promoter

EF1a

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Publication

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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CAS9PBKS

Plasmid

#68371

Purpose

CbAkt promoter driven Cas9-2A-GFP (from px458)

Depositor

Eric-Paul Bennett

Publication

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Tags

2A-EGFP

Expression

Mammalian

Promoter

CbAkt

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependent Cas9-GFP expression

Depositor

Michael Brand

Publication

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid

#68059

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Publication

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Plasmid

#179105

Purpose

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

Use

CRISPR

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Publication

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

35Sp::hCas9

Use

CRISPR

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Publication

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Eef1a-1955/-1>nls::Cas9::nls

Plasmid

#59987

Purpose

Eef1a (EF1alpha) promoter driving nls::Cas9::nls

Depositor

Lionel Christiaen

Publication

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Eef1a (EF1alpha) -1955/-1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Publication

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 VQR

Expression

Mammalian

Mutation

VQR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF1a-FB-dCas9-puro

Plasmid

#100547

Purpose

Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

Depositor

Jian Xu

Publication

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
puromycin

Use

CRISPR

Tags

FLAG, Biotin-acceptor-site (Avitag)

Expression

Mammalian

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Publication

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Promoter

tetON

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Publication

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

Plasmid

#188766

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Fuw-dCas9-Dnmt3a

Plasmid

#84476

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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