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We narrowed to 16,985 results for: She

Showing: 11551 - 11600 of 16985 results

  2. miniCRISPRoff-v1

    Plasmid
    #197030
    Purpose
    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized miniCRISRPoff-v1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197030-plasmid-map-sequence-id-388972

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  5. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  15. Cas12Max

    Plasmid
    #195334
    Purpose
    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized Cas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195334-plasmid-map-sequence-id-383108

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  19. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Use
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    159107-plasmid-map-sequence-id-310025

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  23. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159390-plasmid-map-sequence-id-310625

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  31. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  33. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Use
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  41. pL452-Sf3b1-K700E

    Plasmid
    #90425
    Purpose
    selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90425-plasmid-map-sequence-id-182684

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Showing: 11551 - 11600 of 16985 results