-
Plasmid#112304PurposeCRISPR donor plasmid to tag human transcription factor ZNF434 with GFPDepositorInsertZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF165-donor
Plasmid#112302PurposeCRISPR donor plasmid to tag human transcription factor ZNF165 with GFPDepositorInsertZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA_867
Plasmid#231109PurposeA>G base editorDepositorInsertABE8e-nCas9-SpG-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDB_270
Plasmid#231111PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI-HA
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAZ-SK
Plasmid#73962Purposeself-killing gRNA plasmid pMAZ-SKDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTs
Plasmid#31173DepositorInsertE. coli elongation factor Ts
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PX408 Francisella tularensis subsp. novicida Cas9
Plasmid#68705PurposeFrancisella tularensis subsp. novicida Cas9DepositorInsertFtCas9
UseCRISPRTagsHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHvL1P4GA
Plasmid#112028PurposeExpresses sgRNA in barleyDepositorInsertTaU6-LacZ-sgRNA
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVV651
Plasmid#87631PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA
Plasmid#81254PurposeFor expressing Dox and IPTG inducible stgRNAsDepositorInsertstgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRB1080
Plasmid#71309PurposeExpresses VQR Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVQR Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-gfp
Plasmid#128141PurposeDinoflagelate backbone for gfp expressionDepositorInsertGreen fluorescence protein
UseTagsExpressionBacterialMutationPromoterDinoflagellate potential promotersAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3.-6_human ProCas9Flavi-6
Plasmid#121630PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2
Plasmid#140687Purpose3-color stripe (different sgRNAs)DepositorInsert3-color stripe (different sgRNAs)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRB1083
Plasmid#71480PurposeExpresses VRER Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVRER Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereft-3 (eef-1A.1)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTEAD1-donor
Plasmid#112329PurposeCRISPR donor plasmid to tag human transcription factor TEAD1 with GFPDepositorInsertTEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZBTB12-donor
Plasmid#113802PurposeCRISPR donor plasmid to tag human transcription factor ZBTB12 with GFPDepositorInsertZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
UseCRISPRTagsExpressionMutationrs9267664, rs9267663PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Construct 1 - U6p_20nt1_WT_sgRNA
Plasmid#81245PurposeFor assaying self-targeting activity via Cas9DepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXD1-donor
Plasmid#112350PurposeCRISPR donor plasmid to tag human transcription factor MXD1 with GFPDepositorInsertMXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
UseCRISPRTagsExpressionMutationhg38:2:69937537:A>C,rs3771530PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUSF2-donor
Plasmid#112361PurposeCRISPR donor plasmid to tag human transcription factor USF2 with GFPDepositorInsertUSF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
UseCRISPRTagsExpressionMutationhg38:19:35278400:T>A, hg38:19:35278455:G>CPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF423-donor
Plasmid#113822PurposeCRISPR donor plasmid to tag human transcription factor ZNF423 with GFPDepositorInsertZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SET7-Hygro
Plasmid#104407Purposetransient expression of dCas9-SET7 fusion proteinDepositorInsertSET7 (SETD7 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVV647
Plasmid#87630PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorInsertBxb1 attP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)
Plasmid#160647Purposemodule containing the human codon optimized Cas9 and the BASTA selection markerDepositorInsertBASTA / Cas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnos, P35SAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZBTB11-donor
Plasmid#112348PurposeCRISPR donor plasmid to tag human transcription factor ZBTB11 with GFPDepositorInsertZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
UseCRISPRTagsExpressionMutationhg38:3:101651181:A>TPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3_human ProCas9Flavi
Plasmid#121629PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX379 Legionella pneumophila str. Paris Cas9
Plasmid#68316PurposeLegionella pneumophila str. Paris Cas9DepositorInsertLpCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX391 Sphaerochaeta globus str. Buddy Cas9
Plasmid#68328PurposeSphaerochaeta globus str. Buddy Cas9DepositorInsertSgCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgDedd-1
Plasmid#74435PurposesgRNA expression vector for mouse Dedd gene targeting intron 5DepositorInsertDedd (Dedd Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHK001.2
Plasmid#235450PurposeNOT gate receiver with bs for sgRNA2DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.3
Plasmid#235451PurposeNOT gate receiver with bs for sgRNA3DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.4
Plasmid#235452PurposeNOT gate receiver with bs for sgRNA4DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.5
Plasmid#235453PurposeNOT gate receiver with bs for sgRNA5DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.6
Plasmid#235454PurposeNOT gate receiver with bs for sgRNA6DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-tdTomato
Plasmid#232631Purposecatalytically inactive dCas9 for localization; labeled with tdTomatoDepositorInsertdCas9-tdTomato
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_092
Plasmid#231110PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI
UseCRISPR and LentiviralTagsExpressionMutationD10APromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0
Plasmid#196495PurposeCRISPR tool to target astrocytesDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SV40-iBlue-pA
Plasmid#200234PurposeBackbone for cloning iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-Exo-Blast-BFP
Plasmid#196721PurposeSortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.DepositorInsertCas9-Exo1-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZNF778-donor
Plasmid#112379PurposeCRISPR donor plasmid to tag human transcription factor ZNF778 with GFPDepositorInsertZNF778 homology arms flanking EGFP-IRES-Neo cassette (ZNF778 Human)
UseCRISPRTagsExpressionMutationrs4785627,rs28415940,rs9921361,rs28638280,rs60437…PromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF696-donor
Plasmid#113806PurposeCRISPR donor plasmid to tag human transcription factor ZNF696 with GFPDepositorInsertZNF696 homology arms flanking EGFP-IRES-Neo cassette (ZNF696 Human)
UseCRISPRTagsExpressionMutationrs7386259, rs3750217, rs7387702PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX2-donor
Plasmid#113819PurposeCRISPR donor plasmid to tag human transcription factor TOX2 with GFPDepositorInsertTOX2 homology arms flanking EGFP-IRES-Neo cassette (TOX2 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCFL5-donor
Plasmid#113808PurposeCRISPR donor plasmid to tag human transcription factor TCFL5 with GFPDepositorInsertTCFL5 homology arms flanking EGFP-IRES-Neo cassette (TCFL5 Human)
UseCRISPRTagsExpressionMutationhg38:20:62842215:T>APromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF250-donor
Plasmid#112383PurposeCRISPR donor plasmid to tag human transcription factor ZNF250 with GFPDepositorInsertZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
UseCRISPRTagsExpressionMutationhg38:8:144882271:C>G,hg38:8:144882273:T>CPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF462-donor
Plasmid#112380PurposeCRISPR donor plasmid to tag human transcription factor ZNF462 with GFPDepositorInsertZNF462 homology arms flanking EGFP-IRES-Neo cassette (ZNF462 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF41-donor
Plasmid#112375PurposeCRISPR donor plasmid to tag human transcription factor ZNF41 with GFPDepositorInsertZNF41 homology arms flanking EGFP-IRES-Neo cassette (ZNF41 Human)
UseCRISPRTagsExpressionMutationhg38:X:47447496:G>T, hg38:X:47448269:C>A, h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF197-donor
Plasmid#112357PurposeCRISPR donor plasmid to tag human transcription factor ZNF197 with GFPDepositorInsertZNF197 homology arms flanking EGFP-IRES-Neo cassette (ZNF197 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only