159,332 results

1. HIV-dual-GT

   Plasmid

   #122696

   Purpose

   Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

   Depositor

   Klaus Überla

   Article

   Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

   Inserts

   mTagBFP2
   DsRed-max
   

   Use

   Lentiviral

   Tags

   PEST domain

   Expression

   Mutation

   Promoter

   HIV-1 LTR

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Zebrabow-GateDest

   Plasmid

   #118221

   Purpose

   For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L - ccdB survival cassette
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST17-UbE2A

   Plasmid

   #15780

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2A (UBE2A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-CBE4max-SpRYc-P2A-EGFP

   Plasmid

   #208336

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CAG

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. R-pre-GFP

   Plasmid

   #17274

   Depositor

   Sergio Grinstein

   Article

   Yeung et al Science. 2006 Jul 21. 313(5785):347-51.

   Insert

   R-pre
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pAAV.CMV.ffLuciferase.SV40

   Plasmid

   #105532

   Purpose

   AAV expression of firefly luciferase from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Luciferase
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

   Plasmid

   #174081

   Purpose

   Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
   

   Use

   Tags

   NES-Flag

   Expression

   Mammalian

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pROSA26-ERT2CreERT2

    Plasmid

    #149436

    Purpose

    ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    ERT2-Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF05J

    Plasmid

    #42941

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Negative control Functionally Dead Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    ORF1 R261A, R262A

    Promoter

    Available since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBBR1k-con-GFPuv

    Plasmid

    #106386

    Purpose

    Constitutive trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB4581

    Plasmid

    #171124

    Purpose

    A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    3_sAID
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. peCALWY-4

    Plasmid

    #22236

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-4 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C418S mutation linker consists of 9 GGSGGS repea…

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. CL20_mEGFP_CBFA2T3_GLIS2

    Plasmid

    #205786

    Purpose

    Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXG289

    Plasmid

    #141283

    Purpose

    OE of DELE1

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    DELE1 (DELE1 Human)
    

    Use

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST47-Arl13b12

    Plasmid

    #40872

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptrc

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. LentiGuide Cherry

    Plasmid

    #170510

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Pavel Tolar

    Article

    Newman et al biorXiv 2021.03.02.433398

    Inserts

    S. pyogenes sgRNA cassette
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDNA3 Flag DLC1

    Plasmid

    #24265

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC1 (DYNLL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-C2X dAmp/Kana+

    Plasmid

    #154352

    Purpose

    Empty backbone vector

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Myc-Foxo1 D256

    Plasmid

    #12145

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a deletion truncating the protein after …

    Promoter

    CMV

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Fragment1 with TLS2

    Plasmid

    #196983

    Purpose

    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Intermediate fragment for assembly of gRNA-TLS2 construct
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-mScarlet-Control-5'UTR

    Plasmid

    #184637

    Purpose

    Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SpCas9-CBE6d

    Plasmid

    #215822

    Purpose

    Express CBE6d (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CAG-mGreenLantern

    Plasmid

    #164469

    Purpose

    Constitutive CAG-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CAG

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 RRM2

    Plasmid

    #13796

    Depositor

    Edward Whang

    Article

    Duxbury et al Biochem Biophys Res Commun. 2007 Mar 2.

    Insert

    RRM2 (RRM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-Peredox

    Plasmid

    #73807

    Purpose

    Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

    Depositor

    Gary Yellen

    Article

    Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

    Insert

    Peredox
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA-FLAG-METTL3

    Plasmid

    #160250

    Purpose

    Express FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTSN6

    Plasmid

    #131318

    Purpose

    Integrating the full-length tetO array (from pLAU44) near the his-3 gene in N. crassa

    Depositor

    Eugene Gladyshev

    Article

    Nguyen et al Fungal Genet Biol. 2020 Mar;136:103316.

    Insert

    Full-length tetO array (XbaI-NheI fragment of pLAU44)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge