164,127 results

1. pCMU-GAr

   Plasmid

   #61172

   Purpose

   Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   GmMAN49-mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AP1muA-Halo-ALFA-polyA-G418 HR

   Plasmid

   #229681

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Halo, ALFA

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPT107

   Plasmid

   #85525

   Purpose

   Repressilator with triple reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   mKate2
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVT-eeBxb1

   Plasmid

   #232748

   Purpose

   IVT template for making modRNA encoding eeBxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   V74A, E229K, V375I

   Promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T MDM2 WT

   Plasmid

   #16237

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Tet1

   Plasmid

   #60938

   Purpose

   Expresses Tet1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PA-GFP-α-Tubulin

    Plasmid

    #213900

    Purpose

    Imaging of Tubulin using Photoactivatable GFP

    Depositor

    Lukas Kapitein

    Insert

    PA-GFP-α-Tubulin
    

    Use

    Tags

    Photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid

    #192453

    Purpose

    intronized ttLbCas12a for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    Expression

    Mutation

    temperature-tolerant mutation (D156R)

    Promoter

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ALB-bio-His

    Plasmid

    #52176

    Purpose

    Expresses full-length Serum albumin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ALB (ALB Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIpA102-G-HLA-A2_GFP

    Plasmid

    #85162

    Purpose

    for mRNA expression of HLA.A2-EGFP

    Depositor

    Sébastien Walchli, Tone Fredsvik Gregers

    Article

    Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

    Insert

    HLA.A2 (HLA-A Human)
    

    Use

    Mrna expression

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AYBEv3

    Plasmid

    #193967

    Purpose

    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G163R, N169S, S198A, K202A, G203A, S206A, K210A

    Promoter

    hU6, EF1a, CBh

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKB01_mCherry

    Plasmid

    #69104

    Purpose

    Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZFN-AAVS1-KKR

    Plasmid

    #159298

    Purpose

    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.3-SARS2-B.1.617.2

    Plasmid

    #172320

    Purpose

    expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

    Depositor

    David Nemazee

    Article

    Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

    Insert

    Spike of B.1.617.2 strain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

    Promoter

    CMV-F

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-hRARβ.hRXRα

    Plasmid

    #135415

    Purpose

    Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-beta
    Human retinoid X receptor-alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. P307-FBP_6

    Plasmid

    #139697

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pZFN-AAVS1_ELD

    Plasmid

    #159297

    Purpose

    ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGWB401

    Plasmid

    #74795

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBR_attB(bxb)_ccdB_lox_v2

    Plasmid

    #198040

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Promoter

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT7-TaGu-ENT-3xF-ORF

    Plasmid

    #238488

    Purpose

    For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    R1109->A

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF2158

    Plasmid

    #141949

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCL11B (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge