170,787 results

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GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCR4-24XPP7SL

Plasmid

#31864

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7 Stem Loop Cassette

Available Since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-hnRNPA2B1

Plasmid

#181714

Purpose

Galactose inducible expression of hnRNPA2B1

Depositor

Alejandro Chavez

Insert

hnRNPA2B1 (HNRNPA2B1 Human)

Expression

Yeast

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pSDMA58

Plasmid

#67942

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.

Depositor

James Fraser

Article

Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

Type

Empty backbone

Use

Cryptococcal complementation vector

Promoter

ACT1 for Cryptococcal marker

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-hARL1(WT)

Plasmid

#206406

Purpose

Mammalian expression of hARL1(WT)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUDP025

Plasmid

#103874

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red-C199S

Plasmid

#48252

Purpose

Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED-C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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H-IRF7

Plasmid

#169785

Purpose

Express IRF7 in bacteria

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)

Tags

His tag

Expression

Bacterial

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-126-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85908

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-126-3p Sensor (MIR126 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35 promoter

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1mSMAD

Plasmid

#230917

Purpose

Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)

Use

Luciferase

Tags

Luciferase-luc2p

Expression

Bacterial and Mammalian

Mutation

COL1A1 promoter with mutant potential SMAD bindin…

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-FHC

Plasmid

#64874

Purpose

lentiviral vector with Flag, HA tags

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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reporter-gT1

Plasmid

#47320

Purpose

TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-HA-CYLD

Plasmid

#22544

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pYTK001

Plasmid

#65108

Purpose

Part Plasmid Entry Vector to be used in the Dueber YTK system.

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

none

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAKgfplux1

Plasmid

#14083

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Syn.GCaMP6f.WPRE.SV40

Plasmid

#100837

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-PPARA

Plasmid

#169019

Purpose

Expression of PPARA driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human ppara (PPARA Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS

Plasmid

#29629

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Article

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-LC3B WT

Plasmid

#123092

Purpose

Expresses 3xFLAG-LC3B wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

Plasmid

#21845

Depositor

David Ron

Insert

ATF4 (Atf4 Mouse)

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
hCathepsin B

Plasmid

#11249

Purpose

Mammalian expression of human cathepsin B

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin B (CTSB Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC4A1

Plasmid

#132180

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A1 (SLC4A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-GAL4

Plasmid

#24344

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His6, LgBiT

Expression

Bacterial

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM55273

Plasmid

#153211

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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