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178,697 results

Showing: 7051 - 7080 of 178697 results

  2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99698-plasmid-map-sequence-id-194602

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  3. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Publication
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111090-plasmid-map-sequence-id-264731

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  4. VEGFR2-mCh

    Plasmid
    #108854
    Purpose
    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker
    Depositor
    Kalina Hristova
    Publication
    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KDR (KDR Human)
    Tags
    Labeled with mCherry on the C-Terminus via a 3 am…
    Expression
    Mammalian
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108854-plasmid-map-sequence-id-208010

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  8. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid
    #100844
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CAG promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100844-plasmid-map-sequence-id-461658

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  12. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  14. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Publication
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  19. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Publication
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InsRA (INSR Human)
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  23. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Publication
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70683-plasmid-map-sequence-id-217910

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Showing: 7051 - 7080 of 178697 results