We narrowed to 13,692 results for: EGF

1. pMig (Martins Lab)

   Plasmid

   #14125

   Depositor

   L. Miguel Martins

   Article

   Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

   Insert

   IRES-EGFP
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Cla I sites were silenced in the original pRetroS…

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. iLID-mCherry-iTrkA

   Plasmid

   #136509

   Purpose

   Mammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)

   Depositor

   Bianxiao Cui

   Article

   Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

   Insert

   iLID mCherry iTrkA (Ntrk1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NLS-dCas9-HA-NLS-GFP4-VPR

   Plasmid

   #183924

   Purpose

   VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP4-VPR
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Myc-CD4-mCherry-HA-FRB

   Plasmid

   #100740

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD4
   

   Use

   Tags

   FRB, HA, Myc, and mCherry

   Expression

   Mammalian

   Mutation

   Engineered to remove large part of extracellular …

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCellFree_G03 CTTN

   Plasmid

   #67100

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CTTN (CTTN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLL3.7 shRNA against CRTC1

   Plasmid

   #72710

   Purpose

   Lentiviral expression of shRNA against CRTC1 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC1
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 NFKB1

   Plasmid

   #67126

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pMIEG3-NFAT2

   Plasmid

   #40351

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2006 Jan 15;176(2):880-8.

   Insert

   NFAT2 (NFATC1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

10. NKP33 GFP (1032)

    Plasmid

    #62022

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    C9orf46 (PLGRKT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. N-term Hec1-targeted Aurora B FRET sensor

    Plasmid

    #45239

    Purpose

    sensor targeted to N-terminus of Hec1

    Depositor

    Michael Lampson

    Article

    Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

    Insert

    Hec1, Aurora B FRET sensor
    

    Use

    Tags

    Aurora B FRET sensor and Hec1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. sspa-GCaMP6f

    Plasmid

    #67561

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. px458_2A_GFP_sgRNA_PRKRA

    Plasmid

    #106109

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting PRKRA (PRKRA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 GTF2B

    Plasmid

    #67067

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. ZJOM12

    Plasmid

    #133638

    Purpose

    Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-MuTMC2truncated

    Plasmid

    #154909

    Purpose

    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-MuTMC2truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-179 and 691-716

    Promoter

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCellFree_G03 IFNG

    Plasmid

    #67056

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IFNG (IFNG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pmyc-GFP-TNRC6A-NLS/NES-mut

    Plasmid

    #42002

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCA-T-intron(Neo)-G(FLLFLNL)

    Plasmid

    #40021

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (ATG)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mutation

    deleted nucleotides 1-274 and insertion of FRT, …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCellFree_G03 FYN

    Plasmid

    #67106

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FYN (FYN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pTK157_mCherry-4.1G-CTD

    Plasmid

    #46359

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G C-terminal domain (EPB41L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C-terminal domain from 886-1005 amino acids

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FHp5pUP95C3,5SGW (E5)

    Plasmid

    #74035

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Oliver Schluter

    Article

    Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pLib6.4-Aalb_726

    Plasmid

    #176661

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLib6.4-Aaeg_763

    Plasmid

    #176658

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFasBac+GFP-CAMSAP2 CC+CKK

    Plasmid

    #59039

    Purpose

    for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CC+CKK domains (CAMSAP2 Human)
    

    Use

    Tags

    His and eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46593

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-16 Decoy (Mir16-1 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pCellFree_G03 CREBZF

    Plasmid

    #67070

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CREBZF (CREBZF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR-GFP-Patronin CC+CKK

    Plasmid

    #59048

    Purpose

    for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pENTR-GFP-Patronin CC 534–1457

    Plasmid

    #59050

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pInducer21 Flag-Sesn2

    Plasmid

    #61829

    Purpose

    Dox inducible lentivirial expression of Flag-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pNMHCII-C1C2-GFP

    Plasmid

    #46044

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. UAS-Xbp1-GFP

    Plasmid

    #80627

    Purpose

    expression of Xbp1-GFP (RA isoform) in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    RA isoform

    Promoter

    UAS

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

38. mGFAP R236H

    Plasmid

    #169475

    Purpose

    Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    HaloTAG mGFAP (R236H) (Gfap Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. cmamxA1-GFP

    Plasmid

    #107195

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMTG03-AmpFRT

    Plasmid

    #128272

    Purpose

    TIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-TIP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMTG05-Amp-FRT

    Plasmid

    #128274

    Purpose

    TIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-TIP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMTG04-AmpFRT

    Plasmid

    #128273

    Purpose

    Deg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFP

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Inserts

    TetR-LOV-RRRG
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Py2087ACR1

    Plasmid

    #153032

    Purpose

    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

    Insert

    Py2087ACR1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pGL3-U6-sgRNA_EMX1-mcherry

    Plasmid

    #159784

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human EMX1 gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting EMX1gene (EMX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pJOE6089.4

    Plasmid

    #135081

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-eGFP-Strep-tag II
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBEST-HipO

    Plasmid

    #114599

    Purpose

    Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. OVA-miSFIT-19-G

    Plasmid

    #124676

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCALNL_chr13_102010574-102010650

    Plasmid

    #81213

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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